Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 211 results for: tet rtTA

Showing: 61 - 80 of 211 results

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lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

Plasmid

#229545

Purpose

Lentiviral construct to express rtTA and SKM proteins

Depositor

Domitilla Del Vecchio

Article

Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

Insert

rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hEF1a

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Ngn2_2A_Sox11-PURO

Plasmid

#226284

Purpose

Tetracycline-inducible expression of Ngn2 and Sox11 for direct reprogramming of canine fibroblasts to induced-motor neurons

Depositor

Susannah Sample

Article

Anderson et al STAR Protoc. 2025 Sep 19;6(3):103975. do

Insert

NGN2:T2A:SOX11 (SOX11, NEUROG2 Human)

Use

Lentiviral

Tags

NGN2 and SOX11 linked via T2A

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

Plasmid

#229797

Purpose

Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Isl1_2A_Lhx3-BSD

Plasmid

#226282

Purpose

Tetracycline-inducible expression of Isl1 and Lhx3 for direct reprogramming of canine fibroblasts to induced-motor neurons

Depositor

Susannah Sample

Article

Anderson et al STAR Protoc. 2025 Sep 19;6(3):103975. do

Insert

ISL1:T2A:LHX3 (ISL1, LHX3 Human)

Use

Lentiviral

Tags

Isl1 and Lhx3 linked via T2A

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pHR hEF1A-BFP-P2A-rtTA-WPRE

Plasmid

#223288

Purpose

A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

BFP-P2A-rtTA

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-TRE-CMV-Cre-rtTA

Plasmid

#165457

Purpose

Tetracycline inducible CRE expression from AAVS1 locus

Depositor

Madeline Lancaster

Article

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

CRE

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

Plasmid

#229807

Purpose

A CAGGS driven tet-on transactivator with an nls-EGFP reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rtTA-IRES-nls-EGFP

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

Plasmid

#85798

Purpose

donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235268

Purpose

Lentiviral expression of ComMAND base gene regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-EGFP-FMRP-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

Plasmid

#235306

Purpose

Lentiviral expression of ComMAND base gene regulating EGFP-FMRP (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)

Plasmid

#85799

Purpose

donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235318

Purpose

Lentiviral expression of mRuby2 with 3' microRNA only

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235317

Purpose

Lentiviral expression of mRuby2 with intronic microRNA only

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF4x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235316

Purpose

Lentiviral expression of mRuby2 with 5' microRNA target site only (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-TS.FF6x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235315

Purpose

Lentiviral expression of mRuby2 with 5' microRNA target site only (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-mRuby2-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235313

Purpose

Lentiviral expression of mRuby2 with 3' microRNA target site only (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235314

Purpose

Lentiviral expression of mRuby2 with 3' microRNA target site only (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-FXN-P2A-mRuby2-bGH]-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#235303

Purpose

Lentiviral expression of ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)

Plasmid

#85800

Purpose

donorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2Bneon

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiX1-[TRE3G-TS.FF6x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

Plasmid

#235323

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 61 - 80 of 211 results

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