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pcDNA-HA-Nedd4(C744E)/2797
Plasmid#11206DepositorInsertNedd4-1 (Nedd4 Mouse)
UseTagsHAExpressionMammalianMutationcysteine 744 mutated to glutamic acidPromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCINeo-myc-Itch (C822S)/2860
Plasmid#11428DepositorInsertItch (Itch Mouse)
UseTagsmycExpressionMammalianMutationcysteine at 822 mutated to serinePromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJW1664
Plasmid#112038PurposeCEN/ARS plasmid expression of mitochondrial Su9-TagBFP marked with HISDepositorInsertSu9
UseTagsTagBFPExpressionYeastMutationamino acids 1-76PromoterAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH0007
Plasmid#174162PurposeUCOE-EF1A-Cas9-BFPDepositorInsertCas9-BFP
UseLentiviralTagsExpressionMutationPromoterEF1AAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27A
Plasmid#139193PurposeExpresses C-terminal HA tagged human Mfn2 S27A in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationSerine 27 to AlaninePromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1507
Plasmid#62361PurposepFA6a -mVenus-BirA::HIS5DepositorInsertc-terminal mVenus-BirA tagging
UseYeast c-terminal tagging vectorTagsmVenus-BirAExpressionMutationL232H mutation compared to standard mVenusPromoterAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SARS-CoV-2 Nsp3TMC111A
Plasmid#204207PurposeMammalian expression of SARS-CoV-2 Nsp3DepositorInsertSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
UseTagsFLAGx1ExpressionMammalianMutationCys to Ala in active site of PLproPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SARS-CoV-2 Nsp3TM
Plasmid#204206PurposeMammalian expression of SARS-CoV-2 Nsp3DepositorInsertSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
UseTagsFLAGx1ExpressionMammalianMutationnone, from obtained clonePromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJW1663
Plasmid#112037PurposeGal-Estradiol transcription activation expression cassette marked with KanRDepositorInsertGal DNA binding-estradiol binding-MSN2 activator
UseTagsExpressionYeastMutationPromoterADH1Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1661
Plasmid#112035PurposeOsTIR1 expression cassette marked with URADepositorInsertTIR1
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1666
Plasmid#112040PurposeCEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URADepositorInsertNone
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-mdm2/2702
Plasmid#11492DepositorInsertmdm2 (MDM2 Human)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27D
Plasmid#139194PurposeExpresses C-terminal HA tagged human Mfn2 S27D in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags2HAExpressionMammalianMutationSerine 27 to Aspartic AcidPromoterCMV promoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-human TCR zeta/2470
Plasmid#11507DepositorInsertTCR zeta (CD247 Human)
UseGeneral cloningTagsExpressionMutationn/aPromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mE6-AP/2591a
Plasmid#11468DepositorInsertmE6-AP (Ube3a Mouse)
UseTagsmycExpressionMammalianMutationn/aPromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTMB400_ZFcharm_Prnp_DPM
Plasmid#220847PurposeAAV genome expressing ZFcharm targeting Prnp; double perfect match self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB399_ZFcharm_Prnp_SPM
Plasmid#220846PurposeAAV genome expressing ZFcharm targeting Prnp; single perfect match self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB340_ZFcharm_Kv1_Prnp_DPM
Plasmid#220843PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGM234
Plasmid#220177PurposeExpresses exogenous wild-type NAC complex members (NACA and BTF3)DepositorUseLentiviralTags3xFLAG (NACA); 6xHis (BTF3)ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only
