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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,426 results for: 683

Showing: 781 - 800 of 2426 results

  2. pD649-HAsp-HLA-F-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156834
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    HLA-F (HLA-F Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156834-plasmid-map-sequence-id-302777

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  3. pD649-HAsp-BTN2A2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156683
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    BTN2A2 (BTN2A2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156683-plasmid-map-sequence-id-302475

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  4. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

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  13. pLV[Exp]-Bsd-CMV>hCDK11B D562A

    Plasmid
    #239217
    Purpose
    Expresses CDK11B with D562A kinase inactivating mutation in mammalian cell lines
    Depositor
    Jason Sheltzer
    Article
    Julian et al bioRxiv 2025.08.03.668359; doi: https://
    Insert
    cyclin dependent kinase 11B with D562A kinase inactivating mutation (CDK11B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    changed Aspartic acid 562 to Alanine
    Promoter
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239217-plasmid-map-sequence-id-474408

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  14. pLV[Exp]-Bsd-CMV>hCDK11B-p58 G579S

    Plasmid
    #239216
    Purpose
    Expresses CDK11B p58 isoform with G579S resistance mutation in mammalian cell lines
    Depositor
    Jason Sheltzer
    Article
    Julian et al bioRxiv 2025.08.03.668359; doi: https://
    Insert
    cyclin dependent kinase 11B p58 isoform with G579S resistance mutation (CDK11B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    changed Glycine 579 to Serine
    Promoter
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239216-plasmid-map-sequence-id-474391

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  15. pLV[Exp]-Bsd-CMV>hCDK11B D562A, G579S

    Plasmid
    #239218
    Purpose
    Expresses CDK11B with D562A kinase inactivating mutation and G579S resistance mutation in mammalian cell lines
    Depositor
    Jason Sheltzer
    Article
    Julian et al bioRxiv 2025.08.03.668359; doi: https://
    Insert
    cyclin dependent kinase 11B with D562A kinase inactivating mutation and G579S resistance mutation (CDK11B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    changed Aspartic acid 562 to Alanine and changed …
    Promoter
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239218-plasmid-map-sequence-id-476822

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Showing: 781 - 800 of 2426 results