We narrowed to 156,863 results for: Des

1. pDEST-SorLA-v1

   Plasmid

   #154892

   Purpose

   Expresses SorLA fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   SORL1 (SORL1 Human)
   

   Use

   Tags

   Venus fragment 1 (v1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest17_N-myc

   Plasmid

   #234045

   Purpose

   Bacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage site

   Depositor

   Tanja Mittag

   Article

   Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

   Insert

   N-myc (MYCN Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRT-TO-DEST

   Plasmid

   #106348

   Purpose

   Destination vector for gateway cloning. Developed for Flp-In T-REx system.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Type

   Empty backbone

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT-TODestGFP_RNAseH1

   Plasmid

   #65784

   Purpose

   expresses GFP-tagged RNAseH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Zebrafish, Human)
   

   Use

   Tags

   GFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-FLAG

   Plasmid

   #183511

   Purpose

   Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

   Depositor

   Emanuele Panza

   Article

   Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

   Type

   Empty backbone

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST exorh:mCherry

   Plasmid

   #195989

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann, Nicolas Rohner

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRosa26-DEST

   Plasmid

   #21189

   Purpose

   Rosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in transgenic mice

   Depositor

   Peter Hohenstein, Nick Hastie

   Article

   Hohenstein et al Pathogenetics. 2008 . 1(1):3.

   Insert

   GateWay conversion cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFRT-TODestFLAGHAhFXR1

   Plasmid

   #48694

   Purpose

   Expresses hFXR1

   Depositor

   Thomas Tuschl

   Article

   Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

   Insert

   FlagHA FXR1 (isoform b) (FXR1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available Since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST51-LRRK2-WT

   Plasmid

   #25080

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Destination vector

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX6P1-DEST

    Plasmid

    #119749

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

    Depositor

    Andrew Jackson, Martin Reijns

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST26-N-FLAG

    Plasmid

    #79549

    Purpose

    Destination vector containing an N-terminal FLAG tag

    Depositor

    Rita Shiang

    Article

    Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

    Insert

    FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pMetYC-Dest

    Plasmid

    #105081

    Purpose

    Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

    Depositor

    Christopher Grefen

    Article

    Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

    Type

    Empty backbone

    Use

    Tags

    Cub-PLV

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Desmoglein2-mCherry

    Plasmid

    #36991

    Depositor

    Kathleen Green

    Article

    Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

    Insert

    Dsg2 (DSG2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCVpuro-DEST

    Plasmid

    #119745

    Purpose

    Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Benitez-Guijarro et al EMBO J. 2018 Aug 1;37(15). pii: embj.201

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNX32-Dest

    Plasmid

    #105083

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

    Depositor

    Christopher Grefen

    Article

    Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

    Type

    Empty backbone

    Use

    Tags

    NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-GFP1-10-pDEST-N

    Plasmid

    #118368

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    HA-GFP1-10

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-V1-ORF

    Plasmid

    #73635

    Purpose

    Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDest-eGFP-N1

    Plasmid

    #31796

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST-V2-ORF

    Plasmid

    #73636

    Purpose

    Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 2 (V2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST14-DoubleCatcher

    Plasmid

    #216284

    Purpose

    Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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