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pGEMHE-mEGFP-mTrim21ΔRING-Box

Plasmid

#105520

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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Psicheck2 COLIA1 full length 3'UTR

Plasmid

#26993

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

COL1A1 Full length 3' UTR (COL1A1 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-X1-Neo-GFP-ATL2-R244Q

Plasmid

#109023

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-R244Q (ATL2 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Arg244Gln mutation results in dimerization defect

Promoter

EIF-1a promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAC92-pmax-dCas9VP96

Plasmid

#48224

Purpose

dCas9VP96 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP96 activation domain

Use

CRISPR

Tags

HA Tag and VP96

Expression

Mammalian

Mutation

D10A;H840A

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔPRYSPRY

Plasmid

#105521

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Expression

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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Psicheck2 MERTK full length 3'UTR

Plasmid

#26992

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

MERTK Full length 3' UTR (MERTK Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCRISPRa_all-in-one_Bip gRNA1

Plasmid

#183696

Purpose

Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

gRNA targeting chinese hamster Bip (Hspa5)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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HA-dora[B]_rescue_construct

Plasmid

#190638

Purpose

Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Use

Tags

3xHA

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[A]_rescue_construct

Plasmid

#190640

Purpose

Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Use

Tags

3xHA

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL2-deltaTM

Plasmid

#109021

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-deltaTM (ATL2 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

truncated form without C-terminal ER transmembran…

Promoter

EIF-1a promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dll4CE2-P1Egfp

Plasmid

#90147

Purpose

contains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4E2P1Egfp (dll4 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-1

Plasmid

#109008

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #1 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc

Plasmid

#138368

Purpose

Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dll4CE2-basEgfp

Plasmid

#90144

Purpose

contains dll4 enhancer 2 sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4E2basEgfp (dll4 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG

Plasmid

#69114

Purpose

Dual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
shch-TOG

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

L1939A and L1942A amino acid substitutions. Sile…

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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TOPO mMecp2-NLucTom

Plasmid

#159282

Purpose

To tag endogenously and c-terminally mouse Mecp2 with two reporters: NanoLuciferase and TdTomato. It contains 2 homology arms from a castaneus background surrounding the STOP codon.

Depositor

Joost Gribnau

Article

Mira-Bontenbal et al Stem Cell Reports. 2022 Jan 29. pii: S22

Insert

Mecp2 (Mecp2 Mouse)

Use

Tags

Nluciferase-P2A-TdTomato

Expression

Mammalian

Mutation

Nluciferase-TdTomato fusion

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIP GFP-MOSPD2 WT

Plasmid

#186467

Purpose

Expression of human MOSPD2 fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCGN-ATF6 (1-373)

Plasmid

#27173

Purpose

For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

Depositor

Ron Prywes

Article

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

ATF6 (ATF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Contains only aa 1-373 (please see depositor comm…

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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p026 pCS2-EnR

Plasmid

#11028

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

engrailed (en Fly)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Engrailed repressor domain (bases encoding amino …

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAC2-dual-dCas9VP48-sgExpression

Plasmid

#48236

Purpose

Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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