We narrowed to 2,968 results for: GAI

1. Nme2Cas9_AAV

   Plasmid

   #119924

   Purpose

   All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   AAV

   Tags

   NLS-3xHA-NLS and NLS-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Matrix-roGFP

   Plasmid

   #49437

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   Matrix-roGFP2
   

   Use

   Adenoviral

   Tags

   Cytochrome c oxidase subunit IV mitochondrial tar…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBR1.cas9-NT

   Plasmid

   #197871

   Purpose

   Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

   Depositor

   Baojun Wang

   Article

   Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

   Inserts

   trancCRNA
   Cas9
   crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

   Plasmid

   #227246

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α and pTRE3G

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGMC00028 (aka pTS0057)

   Plasmid

   #200832

   Purpose

   AAVS1 guide RNA cloned into the lentiSAM puro backbone

   Depositor

   Raj Chari

   Article

   Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

   Insert

   AAVS1 guide RNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-BRD4

   Plasmid

   #183939

   Purpose

   Transcriptional co-activator BRD4 labeled by mCherry

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   mCherry-BRD4 (Brd4 Synthetic, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDESTmycIGF2BP3

   Plasmid

   #19879

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Oct 31. ():.

   Insert

   Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-GEM-GECO1

   Plasmid

   #32442

   Purpose

   Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTone-gata2aECE-Kaede

   Plasmid

   #132976

   Purpose

   gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Inserts

   Kaede
   gata2a endothelial enhancer (x6) and a carp b-actin basal promoter
   

   Use

   Unspecified; Transposon-mediated

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX302 OASL-V5 puro

    Plasmid

    #158643

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OASL.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OASL (OASL Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX302 OAS1-V5 puro

    Plasmid

    #158641

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OAS1.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OAS1 (OAS1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-Foxo1-WT 6KR

    Plasmid

    #17560

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 6KR (Foxo1 Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    K242, K245, K259, K262, K271 and K291 were replac…

    Promoter

    CMV

    Available since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

13. p2xFLAGhYAP1

    Plasmid

    #17791

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-Foxo1ADA(pCMV5)

    Plasmid

    #12149

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 ADA (Foxo1 Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Threonine 24 to Alanine (A) and Serine 253 to Asp…

    Promoter

    CMV

    Available since

    June 13, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-CaMKII-jGCaMP7f-WPRE

    Plasmid

    #203848

    Purpose

    Expression of GCaMP7f in CaMKII expressing cells

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    jGCaMP7f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid

    #227245

    Purpose

    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

    Depositor

    Jae Seong Lee

    Article

    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

    Inserts

    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α and pTRE3G

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV asyn WT

    Plasmid

    #36055

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein WT (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQCXIX_sh_HOIP/RNF31_#6

    Plasmid

    #75252

    Purpose

    retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    shRNA against human RNF31 (RNF31 )
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-Foxo1-WT 6KQ

    Plasmid

    #17562

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 6KQ (Foxo1 Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    K242, K245, K259, K262, K271 and K291 were replac…

    Promoter

    CMV

    Available since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pTorPE-R-GECO1

    Plasmid

    #32465

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Lab constructed

    Tags

    6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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