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pBSU6-shTOM40.2

Plasmid

#89882

Purpose

Expresses shRNA against TOM40

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tom40

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pX330.LoxPO

Plasmid

#66585

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pCD294.9

Plasmid

#113319

Purpose

dCas9, PCP-SoxS_R93A, J109 scRNA.b1

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, PCP-SoxS_R93A, J109 scRNA.b1_PP7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

R93A

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfRab25_4

Plasmid

#26712

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Canine Rab25 RNAi.

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pQdCas12a.luxR(mut)-sggfp(A)

Plasmid

#236039

Purpose

The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

Depositor

Radhakrishnan Mahadevan

Insert

quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene

Use

CRISPR

Tags

Expression

Mutation

Promoter

quorum sensing promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pABE-dGFP-gRNA

Plasmid

#226864

Purpose

Human gRNA expression vector targeting the A111V mutation of non-fluorescent EGFP in plasmid pABE-dGFP; for use with ABEs

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

Sp-gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCBE-dGFP-gRNA

Plasmid

#226862

Purpose

Human gRNA expression vector targeting the Y93H mutation of non-fluorescent EGFP in plasmid pCBE-dGFP; for use with CBEs

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

Sp-gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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p105_gRNA_Sa_CD40

Plasmid

#213781

Purpose

Expresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sa gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

mU6

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-ERCC1 gRNA2

Plasmid

#211631

Purpose

sgRNA-2 against ERCC1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDT4

Plasmid

#199120

Purpose

scrambled promoter circular DNA template

Depositor

Jeff Gelles

Article

Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

Insert

synthetic transcription template

Use

Tags

Expression

Bacterial

Mutation

mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

Promoter

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-gR085

Plasmid

#188554

Purpose

Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR085

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-gR085

Plasmid

#188552

Purpose

Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR085

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgDcc

Plasmid

#159906

Purpose

Mutagenesis of Dcc

Depositor

Larry Zweifel

Insert

Dcc (Dcc Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_THRA_iso1_2

Plasmid

#128246

Purpose

Encodes gRNA for 3' target of human THRA_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

THRA_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB49_iso1_1

Plasmid

#128250

Purpose

Encodes gRNA for 3' target of human ZBTB49_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB49_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB49_iso1_2

Plasmid

#128251

Purpose

Encodes gRNA for 3' target of human ZBTB49_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB49_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSLHDR_R1

Plasmid

#124209

Purpose

gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pWT029g

Plasmid

#96860

Purpose

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT029h

Plasmid

#96861

Purpose

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBbA5k-AcrB/AcrD/AcrFa, ABO_0964

Plasmid

#86202

Purpose

Expresses efflux pump in E. coli to provide resistance to biofuel (medium copy)

Depositor

Mary Dunlop

Article

Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

Insert

Efflux pump

Use

Tags

Expression

Bacterial

Mutation

Promoter

ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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