Addgene

Showing: 781 - 800 of 1513 results

Results per page:

Sort results by:

pBa.Halo-LDLR

Plasmid

#162717

Purpose

Mammalian expression of human LDLR

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

LDLR (LDLR Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

none

Promoter

Chicken Beta actin

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN_U6-TRAC-miniGag

Plasmid

#228960

Purpose

miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIpA102-G-HLA-A2_GFP

Plasmid

#85162

Purpose

for mRNA expression of HLA.A2-EGFP

Depositor

Sébastien Walchli, Tone Fredsvik Gregers

Article

Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

Insert

HLA.A2 (HLA-A Human)

Use

Mrna expression

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IBU-SNAP-Rab7

Plasmid

#228141

Purpose

Expresses SNAP-Rab7 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Rab7 (Rab7 Mouse)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-CD63-FKBP-Myc

Plasmid

#177910

Purpose

human CD63-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD63 (CD63 Human)

Use

Tags

FKBP and Myc tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
I48 ELP

Plasmid

#68937

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

Depositor

Andrew MacKay

Article

Shi et al J Control Release. 2013 Nov 10;171(3):33

Insert

I48 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFsynW SYT1 reporter

Plasmid

#197275

Purpose

Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pF(UG) hSyn SYT7alpha-HaloTag

Plasmid

#179715

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1-sfGFP-DQNAT

Plasmid

#198210

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_DQNAT

Use

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFsynW SYB2 reporter

Plasmid

#197276

Purpose

Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYB2 and HaloTag (Vamp2 Rat)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa.GFP-KIF13B

Plasmid

#134626

Purpose

Mammalian expression of mouse KIF13B 442-1843

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13B (Kif13b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS-HsProm1s1-StayGold

Plasmid

#211333

Purpose

Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

AAV

Tags

StayGold

Expression

Mutation

Promoter

EF1a

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-HiBiT-CD63

Plasmid

#162591

Purpose

N-terminally HiBiT-tagged human CD63 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD63 (CD63 Human, Synthetic, human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
σ1-mCherry

Plasmid

#186578

Purpose

Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP1S1 (AP1S1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
gWiz_BM40-vGAT-VL-hIgG1-CL

Plasmid

#218437

Purpose

mammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domain

Depositor

Axel Brunger

Article

Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

Insert

vGAT-VL-hIgG1-CL

Use

Affinity Reagent/ Antibody

Tags

BM40

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-TPD54

Plasmid

#172448

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

Insert

TPD54 (TPD52L2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

Plasmid

#168216

Purpose

Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

SV40 NLS SpCas9

Use

Lentiviral

Tags

HA, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

Plasmid

#86424

Purpose

PCR tagging module with yeast codon optimized mTurquoise2

Depositor

Sue Jaspersen

Article

Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

Insert

mTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAD-CMV-STAT5A-CA-CMV-GFP

Plasmid

#83255

Purpose

Adenoviral expression of constitutively active STAT5A with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Tags

Expression

Mutation

constitutively active

Promoter

CMV

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge