We narrowed to 2,466 results for: crispr system

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pcDNA-Flag-LmoCas5

Plasmid

#126484

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas8b2

Plasmid

#126488

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas8b2

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas7

Plasmid

#126489

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas7

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas5

Plasmid

#126490

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6

Plasmid

#126491

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas6

Plasmid

#106274

Purpose

Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasE

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-LmoCas6-KRAB-2a-BlastR

Plasmid

#126492

Purpose

Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6-KRAB-2a-BlastR

Use

CRISPR and Lentiviral

Tags

Flag, NLS

Expression

Mammalian

Promoter

hUbC

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas8e

Plasmid

#106270

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasA

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCse2

Plasmid

#106271

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasB

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas7

Plasmid

#106272

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasC

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-EcoCas5

Plasmid

#106273

Purpose

Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasD

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCas3cRh

Plasmid

#133773

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Joseph Bondy-Denomy

Article

Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8
Cas7

Expression

Bacterial

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHS1801

Plasmid

#205966

Purpose

JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH proteins and CRISPR array

Expression

Bacterial

Promoter

Lac (proteins), pJ23119 (crRNA)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pM378

Plasmid

#173174

Purpose

Expresses C9-1 gRNA-12xMBS from hU6 promoter

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

C9-1 targeting gRNA-12xMBS

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ132B2.0

Plasmid

#99888

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ131B2.0

Plasmid

#99885

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

Plasmid

#132405

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176268

Purpose

Expression of dCas12f-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCas12f

Use

Lentiviral

Tags

HA-VPR-mCherry

Expression

Mammalian

Mutation

D326A, D510A

Promoter

PGK

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ132A2.0

Plasmid

#99887

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ133A2.0

Plasmid

#99891

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge