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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,221 results for: sumo

Showing: 781 - 800 of 1221 results

  1. TBXTA-c021

    Plasmid
    #139758
    Purpose
    Protein expression/crystallography (PDB:5QT0)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-N211
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139758-plasmid-map-sequence-id-296541

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  6. CNN3A-c002

    Plasmid
    #175456
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-Y329.
    Depositor
    Opher Gileadi
    Insert
    CNN3 (CNN3 Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175456-plasmid-map-sequence-id-353174

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  7. CNN3A-c007

    Plasmid
    #175457
    Purpose
    Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.
    Depositor
    Opher Gileadi
    Insert
    CNN3 (CNN3 Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175457-plasmid-map-sequence-id-353125

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  8. pET29a-human histone 4-in-One

    Plasmid
    #223237
    Purpose
    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4
    Depositor
    Weixing Zhao
    Inserts
    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    Use
    Tags
    Dual StrepII, His6, TEV protease site, and VSVG
    Expression
    Bacterial
    Mutation
    A103G
    Promoter
    T7
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223237-plasmid-map-sequence-id-443806

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  9. pALS3-Ma-sfGFP-WT

    Plasmid
    #212119
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212119-plasmid-map-sequence-id-444185

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  10. Cys-3HB-SPYcatcher

    Plasmid
    #214838
    Purpose
    This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    Cys-3HB-SPYcatcher
    Use
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214838-plasmid-map-sequence-id-425316

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  11. Cys-60nmSAH-SPYcatcher

    Plasmid
    #214840
    Purpose
    This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    Cys-60nmSAH-SPYcatcher
    Use
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214840-plasmid-map-sequence-id-425312

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  12. SPYtag-GFPnanobody

    Plasmid
    #214837
    Purpose
    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    SPYtag-GFPnanobody
    Use
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214837-plasmid-map-sequence-id-425310

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  13. Cys-30nmSAH-SPYcatcher

    Plasmid
    #214839
    Purpose
    This plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    Cys-30nmSAH-SPYcatcher
    Use
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214839-plasmid-map-sequence-id-425308

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  15. 6CKO (DOT1L)

    Plasmid
    #119962
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Use
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 1, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119962-plasmid-map-sequence-id-234358

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  18. 6CCR (GID4)

    Plasmid
    #119960
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    GID4 (C17orf39 Human)
    Use
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acids 116-300
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119960-plasmid-map-sequence-id-232678

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Showing: 781 - 800 of 1221 results