164,577 results

1. U6-sgfiller-eCas9-T2A-BlastR

   Plasmid

   #172437

   Purpose

   Expresses eCas9, BlastR and sgRNA cloning site

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Inserts

   eCas9
   BlastR
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2_8xCTS-ECFP

   Plasmid

   #200249

   Purpose

   Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   8xCTS-ECFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDM304_DPAKa(GBD)-YFP

   Plasmid

   #128699

   Purpose

   expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

   Depositor

   Igor Weber

   Article

   Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

   Insert

   GBD domain (aa 731-890) of DPAKa
   

   Use

   Dictyostelium discoideum expression vector

   Tags

   YFP

   Expression

   Mutation

   none

   Promoter

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE30-HyPer7

   Plasmid

   #141071

   Purpose

   Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9-miniTurbo

   Plasmid

   #219822

   Purpose

   EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

   Depositor

   Philipp Maass

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   miniTurbo

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   EF1a

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3XFLAG-TAK1-K63A

   Plasmid

   #43933

   Depositor

   Bryant Darnay

   Article

   Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

   Insert

   Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   K63A

   Promoter

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pREP3-TEV-NLS

   Plasmid

   #21146

   Depositor

   Stephen Kearsey

   Article

   Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

   Insert

   TEV protease (TEVgp1 tobacco etch virus)
   

   Use

   S. pombe

   Tags

   2xNLS, NLS, and myc

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2R3a-GUS-OcsT

   Plasmid

   #71267

   Purpose

   Entry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   Beta-glucuronidase
   

   Use

   Gateway

   Tags

   2xGly linker and octaline synthase terminator

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRE-Tn5-161

   Plasmid

   #118541

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPI356

    Plasmid

    #207449

    Purpose

    Extrachromosomal expression vector PkgE-PH-GFP and PTEN-mCherry

    Depositor

    Rob Kay

    Article

    Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

    Inserts

    PkgE
    PTEN
    

    Use

    Unspecified

    Tags

    GFP and mCherry

    Expression

    Mutation

    PH domain only

    Promoter

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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