162,094 results

1. R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166014

   Purpose

   mammalian expression of HCoV-HKU1 soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   HCoV-HKU1 S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-PLK2

   Plasmid

   #23691

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PLK2 (PLK2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. Ef1a_Large T-antigen_Ires_Puro

   Plasmid

   #18922

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   Large T Antigen (SV40gp6 Simian virus 40)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. NeonGreen-C1-ORP5

   Plasmid

   #220071

   Purpose

   overexpresses ORP5 with a neonGreen fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL5 (OSBPL5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDN77

   Plasmid

   #61347

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

   Depositor

   Roland Eils, Barbara Di Ventura

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS2 and NES IkBa

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

   Plasmid

   #132410

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dEsCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R295A; H300A; R849A; H854A

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mNeonGreen-APEX3

   Plasmid

   #202590

   Purpose

   Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

   Depositor

   Reuben Harris

   Article

   Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

   Insert

   mNeonGreen-APEX3-V5 (APX1 )
   

   Use

   Retroviral

   Tags

   V5 epitope and mNeonGreen

   Expression

   Mammalian

   Mutation

   APEX2 L242A

   Promoter

   MESV LTR

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ube1/PET21d

   Plasmid

   #34965

   Depositor

   Cynthia Wolberger

   Article

   Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

   Insert

   Ube1 (UBA1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-CBE4max-SpRYc-P2A-EGFP

   Plasmid

   #208336

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

   Depositor

   Pranam Chatterjee

   Article

   Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

   Insert

   human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Sc++ (1-1119), SpRY (1111-1368), D10A

   Promoter

   CAG

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_GEM2-Halo-FRB

    Plasmid

    #197056

    Purpose

    Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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