164,577 results

1. pDM1383

   Plasmid

   #207423

   Purpose

   Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

   Depositor

   Rob Kay

   Article

   Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

   Inserts

   HSPC300
   Raf1 (RAF1 Human)
   

   Use

   Unspecified

   Tags

   GFP and mCherry

   Expression

   Mutation

   aa 1–134 (RBD) only

   Promoter

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. hsDCX-EGFP-pHAT

   Plasmid

   #83918

   Purpose

   human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

   Depositor

   Gary Brouhard

   Article

   Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

   Insert

   Doublecortin isoform b (DCX Human, NM_178152.2)
   

   Use

   Tags

   6-His and EGFP+Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. XX01CAPN1A-c001

   Plasmid

   #175491

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   CAPN1 / CAPNS1 (CAPN1 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-eGFP-pA

   Plasmid

   #107281

   Purpose

   donor vector backbone with constitutive eGFP

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF-G1mCNPRS

   Plasmid

   #174718

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-mir451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164792

   Purpose

   Express miR-451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   microRNA 451a (MIR451A Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRE-Tn5-160

   Plasmid

   #118540

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   TagBFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. STAT3-GFP

   Plasmid

   #110495

   Purpose

   reporter of STAT3 signaling activity

   Depositor

   Michael Lewis

   Article

   Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

   Insert

   4 repeats of STAT3 binding site M67 at GFP promoter region
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUBC-SV40NLS-Pfv-Sapphire-PURO

   Plasmid

   #203654

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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