Addgene

We narrowed to 15,557 results for: LEA

Showing: 8041 - 8060 of 15557 results

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pGL4.23-IL2RA CaRE6 (minus strand)

Plasmid

#91840

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0617)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE6 (minus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

rs7893467 C>A

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE6 (plus strand)

Plasmid

#91839

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0616)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE6 (plus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

rs7893467 G>T, rs11256448 A>G

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE2 (plus strand)

Plasmid

#91848

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0625)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE2 (plus strand) (CD69 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE2 (minus strand)

Plasmid

#91843

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0620)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE2 (minus strand) (CD69 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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zebrafish similar to gpr151 (or gpcr-2037)_L (OZ535)

Plasmid

#27212

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
zebrafish similar to gpr151 (or gpcr-2037)_R (OZ536)

Plasmid

#27213

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) (LOC565170 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

Plasmid

#223065

Purpose

Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1

Use

CRISPR

Tags

NLS(SV40)-3xFLAG

Expression

Bacterial

Mutation

Three regions of SpCas9 encode type IIS restricti…

Promoter

Dual T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

Plasmid

#223127

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 (plus strand)

Plasmid

#91850

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0345)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 (plus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-IL2RA CaRE4 scramble (plus strand)

Plasmid

#91852

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0347)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 scramble (plus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Sequence scrambled from ch10:6094588-6094934

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 (minus strand)

Plasmid

#91849

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0626)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 (minus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-IL2RA CaRE1 (plus strand)

Plasmid

#91837

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0614)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE1 (plus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B

Plasmid

#133308

Purpose

Expression of luciferase driven by eIF3B promoter region

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter (EIF3B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

eIF3B

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE5 (plus strand)

Plasmid

#91841

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0618)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE5 (plus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-IL2RA CaRE3 (minus strand)

Plasmid

#91835

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0612)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE3 (minus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

rs7893324 A>G, rs7090530 G>T, rs7090512 A&g…

Promoter

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-IL2RA CaRE4 SNP (plus strand)

Plasmid

#91851

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0346)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 SNP (plus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

rs61839660 C>T

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-IL2RA CaRE5 (minus strand)

Plasmid

#91836

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0613)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE5 (minus strand) (IL2RA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(MRKQKC)-P2A-EGFP (AHK574)

Plasmid

#223134

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MRKQKC)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MRKQKC)=D1135M, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(MRKSKC)-P2A-EGFP (AHK446)

Plasmid

#223135

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(MRKSKC)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/MRKSKC)=D1135M, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-TadCBEd-SpCas9(KWRQLC)-P2A-EGFP (BKS1026)

Plasmid

#223126

Purpose

pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA-CDd-SpCas9(KWRQLC)-2xUGI-P2A-EGFP

Use

CRISPR

Tags

2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

Expression

Mammalian

Mutation

TadA-CDd mutations; nSpCas9(D10A/KWRQLC)=D1135K, …

Promoter

CMV and T7

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge