166,289 results

1. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

   Plasmid

   #232183

   Purpose

   Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6, hUbC

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCWXPG-UBI-Ngn3PDX1MafA

   Plasmid

   #114303

   Purpose

   Constitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptides

   Depositor

   Patrick Salmon

   Article

   Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

   Insert

   Ngn3 (NEUROG3 Human)
   

   Use

   Lentiviral

   Mutation

   Ngn3, PDX1 and MafA proteins are expressed from a…

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. ER-P-TAG (pGS594)

   Plasmid

   #160039

   Purpose

   pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

   Depositor

   David Sabatini

   Insert

   CP450TMD-APEX2-3xHA
   

   Use

   Lentiviral

   Promoter

   EF1

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-mCherry-(F30-2xPepper)10

   Plasmid

   #129401

   Purpose

   mCherry mRNA reporter tagged with (F30-2xPepper)10

   Depositor

   Samie Jaffrey

   Article

   Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

   Insert

   mCherry-(F30-2xPepper)10
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHSE401

   Plasmid

   #62201

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVITRO1-Trastuzumab-IgG1/κ

   Plasmid

   #61883

   Purpose

   Expresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
   HER2/neu receptor specific humanized kappa light chain expression cassette
   

   Expression

   Mammalian

   Promoter

   mEF1 Prom and rEF1 Prom

   Available Since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSET-dCas9

   Plasmid

   #110183

   Purpose

   Expression of the dCas9 gene in Streptomyces

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Insert

   dCas9
   

   Use

   CRISPR; I

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   ermE*p

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV Rnf121-V5

   Plasmid

   #175130

   Purpose

   Lentiviral expression of mouse Rnf121-V5

   Depositor

   Larry Gerace

   Insert

   Rnf121 (Rnf121 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDH5-AbR-GFP

   Plasmid

   #122970

   Purpose

   Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

   Depositor

   Budd A. Tucker

   Inserts

   CDH5p
   CopGFP
   Zeocin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PT7lacO-RF1-His6

    Plasmid

    #224372

    Purpose

    Express RF1 protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    RF1 (prfA E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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