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pAAV-short Cre-on G/W

Plasmid

#86949

Purpose

Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAV

Depositor

Joseph Dougherty, Rob Mitra

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h

Plasmid

#73066

Purpose

expression clone for human SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD27 Human)

Tags

no tag

Expression

Mammalian

Mutation

expression cassette for SNORD27

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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mitoTALECD for_atp1_1397CN

Plasmid

#186203

Purpose

For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

Depositor

Shin-ichi Arimura

Article

Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

Inserts

mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)

Use

Synthetic Biology; Ti plasmid for plant transform…

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBRPyCAG-fmKlf4-DsRed-Ip

Plasmid

#26274

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Klf4 (Klf4 Mouse)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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His-GST-Cys-M87 Spastin

Plasmid

#184804

Purpose

Bacterial expression of M87 Spastin (A2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

M87 Spastin (SPAST Human)

Tags

His6-GST

Expression

Bacterial

Mutation

A2C

Promoter

T7

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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HOXB7 (human) pCMV

Plasmid

#8539

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
GFP-CLCb (EED/QQN)

Plasmid

#47422

Depositor

Peter McPherson

Article

Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

Insert

CLCb EED/QQN (CLTB Human)

Tags

EGFP

Expression

Mammalian

Mutation

EED changed to QQN, in aa20-41 region

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pDN-G1TGt

Plasmid

#44508

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
tetR::yEGFP

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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PB-hSyn-mSyp-RT1-24xGCN4

Plasmid

#106300

Purpose

The plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.

Depositor

Zhe J. Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

mSyp-RT1-24xGCN4 (Syp Mouse)

Tags

24xGCN4

Expression

Mammalian

Promoter

human Synapsin1

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) FLAG MSCV

Plasmid

#8528

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Missing N-terminal 14 amino acids; improved Ribos…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only