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pAAV_hSyn_NES-Kinprola_PKA-mEGFP_WRPE-SV40

Plasmid

#233366

Purpose

hSyn1 driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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B2342_BTB-mCherry

Plasmid

#215095

Purpose

Expresses BCL6 BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB (BCL6 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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CA-0039 CMVTO-hIL10 (Fused Dimer)

Plasmid

#231187

Purpose

Fused human IL-10 dimer

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Fused human IL-10 dimer (IL10 Human)

Expression

Mammalian

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pD2529-CAG-HA-mouse GARP

Plasmid

#180258

Purpose

Mouse full length GARP expression

Depositor

Timothy Springer

Article

Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

Insert

GARP (Lrrc32 Mouse)

Tags

HA

Expression

Mammalian

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgs2_B6

Plasmid

#170300

Purpose

A knock-out vector for the mouse Ptgs2 in C57BL6

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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AXBE

Plasmid

#204605

Purpose

Mammalian expression of adenine transversion base editor AXBE

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

S. pyogenes Cas9 (D10A)

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGST2-GST-TEV-OPTN

Plasmid

#198428

Purpose

Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

optineurin (OPTN Human)

Tags

GST and TEV

Expression

Bacterial

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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BtuF

Plasmid

#92209

Purpose

Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter

Depositor

Douglas Rees

Article

Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99

Insert

BtuF (btuF )

Tags

6x His Tag and PelB Signal Sequence

Expression

Bacterial

Mutation

BtuF signal sequence removed and uses the PelB si…

Promoter

T7 Promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-SomArchon

Plasmid

#126942

Purpose

Expression of SomArchon under CaMKII promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-CI-SaCas9-W3SynpA

Plasmid

#231369

Purpose

minBG-driven SaCas9, with short synthetic terminator sequence.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

SaCas9

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.minCMVe-SCP1-SaCas9-W3SynpA

Plasmid

#231370

Purpose

SCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

SaCas9

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(H75E)

Plasmid

#137829

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(H75E)

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLXSN-Axl

Plasmid

#65222

Purpose

Expression of Axl in mammalian cells

Depositor

Axel Ullrich

Article

Zhang et al Cancer Res. 2008 Mar 15;68(6):1905-15. d

Insert

AXL (AXL Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-haA3A-CBE-A

Plasmid

#220892

Purpose

Express haA3A-CBE-A (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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[pSK468] pLJC5 Flag-Depdc5

Plasmid

#109337

Purpose

Lenti-viral expression of Flag-tagged wildtype Depdc5

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSLS.419

Plasmid

#187208

Purpose

Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco4 ncRNA
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G_ManII-LaccID-V5_Puro

Plasmid

#234051

Purpose

expresses LaccID in the Golgi apparatus, dox-inducible lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

ManII-LaccID

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3241 PB-AtoBpegRNA-mCherry

Plasmid

#226666

Purpose

PiggyBac cargo vector encoding A to B pegRNA marked with mCherry

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

AtoB pegRNA

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_Kinprola_PKA-mEGFP-MTBD

Plasmid

#233363

Purpose

CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP, targeting to the microtubule

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

Kinprola_PKA-mEGFP-MTBD

Tags

mEGFP-MTBD

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2hyg-Ptgs1

Plasmid

#170299

Purpose

A knock-out vector for the mouse Ptgs1

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Ptgs1 gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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