We narrowed to 42,558 results for: ANT

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pDx_mRuby3-SYFP2

Plasmid

#189758

Purpose

Dual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mRuby3-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMRXIP 3xMyc-STX17ΔNTD

Plasmid

#89940

Purpose

Expresses syntaxin17 lacking N-terminal domain tagged with Myc in mammalian cells

Depositor

Noboru Mizushima

Article

Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

Insert

human syntaxin17 lacking N-terminal domain (STX17 Human)

Use

Retroviral

Tags

Myc

Mutation

deleted amino acids 1-161

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGREAT26

Plasmid

#170914

Purpose

GoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pEstradiol_Inducible

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pHLP701

Plasmid

#203743

Purpose

C-terminal tagging of target proteins with 3xV5-IAA17 degron

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

IAA17 auxin binding domain

Use

Pcr amplification template for gene tagging

Tags

3xV5

Promoter

none

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM35171

Plasmid

#153222

Purpose

Level 1 position 1 part containing the Nos promoter, the BAR gene, and the Ocs terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Nos promoter, BAR gene, Ocs terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAcrVIB1

Plasmid

#184566

Purpose

Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

anti-CRISPR protein inhibiting Cas13b nucleases

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE476

Plasmid

#153267

Purpose

M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-G97

Plasmid

#130705

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE657

Plasmid

#153255

Purpose

M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBacT7-S1T1L

Plasmid

#33292

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S1 (MoV1sS1gp2, MoV1sS1gp1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pDGE656

Plasmid

#153254

Purpose

M2 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLuciferase (GB0096)

Plasmid

#68201

Purpose

Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luciferase coding region

Use

Luciferase and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCyterm-mScarlet-I3-NCwt_N1

Plasmid

#189779

Purpose

Construct for testing oligimerization of mScarlet-I3-NCwt in mammalian cells by OSER assay

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

Cyterm-mScarlet-I3-NCwt

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBK1290-AAV-dSaCas9

Plasmid

#223139

Purpose

AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-ABE4

Plasmid

#195370

Purpose

dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

ecTadA8e-(GGGGS)x6-dLbCas12a-D156R

Use

CRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…

Tags

5' and 3' NLS

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pZeo-Cas9-gate

Plasmid

#113744

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR and Gateway Cloning

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDGE331

Plasmid

#153240

Purpose

M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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L2-AA007

Plasmid

#185750

Purpose

Vector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Inserts

HygR2
eGFP

Tags

membrane localization tag Lti6b

Expression

Plant

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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