We narrowed to 9,980 results for: Uty

1. Bxb1-GT_CAG-ITPKA-mScarlet-I

   Plasmid

   #229778

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mScarlet-I
   

   Use

   Synthetic Biology

   Tags

   ITPKA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

   Plasmid

   #229800

   Purpose

   Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mGreenLantern; mRuby2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   hEF1a and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIplac128-TEF2p-LDH-CYC1t

   Plasmid

   #216120

   Purpose

   Yeast integrative vector for constitutive expression of LDH at the LEU2 locus

   Depositor

   Yvonne Nygård

   Article

   Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

   Insert

   Lactate dehydrogenase (LDH Bovine)
   

   Expression

   Yeast

   Promoter

   TEF2 promoter

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. YIplac204-TDH3p-LDH-CYC1t

   Plasmid

   #216121

   Purpose

   Yeast integrative vector for constitutive expression of LDH at the TRP1 locus

   Depositor

   Yvonne Nygård

   Article

   Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

   Insert

   Lactate dehydrogenase (LDH Bovine, Bos taurus)
   

   Expression

   Yeast

   Promoter

   TDH3 promoter

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. JD179

   Plasmid

   #229496

   Purpose

   Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I gene

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   E2A, L4-22k/33k, VA RNA I
   

   Use

   AAV and Adenoviral

   Expression

   Mammalian

   Promoter

   Tre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHelper-CRISPRi-sgRNA

   Plasmid

   #221135

   Purpose

   Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   sgRNA-scaffold with dCas9 handle
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119(SpeI)

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB4668

   Plasmid

   #215272

   Purpose

   Module for the expression of the N. nambi HISPS gene under the ABA- responsive MAPKKK18 promoter and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   pMAPKKK18:HISPS.TNOS-SF-CPH-SF-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GB4655

   Plasmid

   #215268

   Purpose

   Module for the copper and PhiC31 regulated expression of HispS and the constitutive expression of CPH, LUZ, H3H and eGFP.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   CBS-att-OCS-HispS-tNos-SF-CPH-SF-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. GB4404

   Plasmid

   #215255

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of PNtA11 and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   SF-PNtA11:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GB4403

    Plasmid

    #215254

    Purpose

    Module for the expression of the N. nambi HISPS gene under the regulation of PNtA1 and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    SF-PNtA1:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p8215 pHAGE N-V5 PTPN14 ΔPPXY1/2

    Plasmid

    #221646

    Purpose

    Constitutive ectopic expression of human PTPN14 ΔPPXY1/2 mutant

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    PTPN14 ΔPPXY1/2 (PTPN14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    deletion of the first and second (tandem) PPXY mo…

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p8180 pHAGE N-V5 PTPN14 ΔPPXY3/4

    Plasmid

    #221647

    Purpose

    Constitutive ectopic expression of human PTPN14 ΔPPXY3/4 mutant

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    PTPN14 ΔPPXY3/4 (PTPN14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    deletion of the third and fourth PPXY motifs as d…

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p8189 pHAGE N-V5 PTPN14 C1121S

    Plasmid

    #221648

    Purpose

    Constitutive ectopic expression of human PTPN14 C1121S mutant

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    PTPN14 C1121S (PTPN14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    C1121S mutation at the putative catalytic site

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GB1531

    Plasmid

    #215242

    Purpose

    TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    PNos:PhiC31:TNos
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_575

    Plasmid

    #216076

    Purpose

    Piggybac, constitutive expression destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Piggybac, destination

    Expression

    Mammalian

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CASI-inteinC-aa713 SpG C-U6- sgRNA scaffold

    Plasmid

    #208111

    Purpose

    Expresses SpG cas9C by the constitutive CASI promoter and sgRNA scaffold by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

    Insert

    SpG C, U6, scaffold
    

    Use

    AAV

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC Destination

    Plasmid

    #207675

    Purpose

    Trio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).

    Depositor

    Ramon Gonzalez

    Article

    Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    CymRC, PhlF, VanCC

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT-TO-NUbo-E3(63)-FLAG

    Plasmid

    #212808

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(63)-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Tags

    FLAG and NUbo

    Expression

    Mammalian

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT-TO-NUbo-FLAG

    Plasmid

    #212797

    Purpose

    Constitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe-Sik-YF

    Plasmid

    #210723

    Purpose

    Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

    Depositor

    Angela Tyner

    Article

    Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

    Insert

    Protein tyrosine kinase 6 (Ptk6 Mouse)
    

    Expression

    Mammalian

    Mutation

    Tyrosine 447 changed to Phenylalanine

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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