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Dhh1

Plasmid

#201412

Purpose

Bacterial protein expression of Dhh1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dhh1 (DHH1 Budding Yeast)

Tags

MBP, TEV and TEV, 6xHis

Expression

Bacterial

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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GST-TAX1BP1

Plasmid

#171423

Purpose

For the purifcation of TAX1BP1 protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

TAX1BP1 (TAX1BP1 Human)

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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N-GFP-Dcp2

Plasmid

#163585

Purpose

express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

DCP2 promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMC-1-ZmPLTP-6

Plasmid

#197713

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmPLTP promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-rsTagRFP

Plasmid

#31927

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Tags

His

Expression

Bacterial

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

araB

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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TR-Csy4

Plasmid

#80601

Purpose

Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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SurA

Plasmid

#213138

Purpose

Expresses chaperone SurA

Depositor

Sebastian Hiller

Article

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

SurA (21-428) (surA E.coli)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-9-OsAct2-11

Plasmid

#197765

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

OsAct2 terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-sfGFP

Plasmid

#180757

Purpose

pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-sfGFP-deadTET1CD

Plasmid

#184442

Purpose

Catalytically DEAD TET1CD with scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

Catalytically DEAD TET1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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Phsp-16 cz::caspase-3 (p17) [TU#823]

Plasmid

#16086

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

cz :: caspase-3 (p17) (CASP3 Human)

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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TRPML2-YFP

Plasmid

#18830

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

Plasmid

#199482

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEvoltac EcW-TGA-h9

Plasmid

#218765

Purpose

encodes EcWRS-h9 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h9

Expression

Bacterial

Mutation

S8A, V144S, V146A

Promoter

tacI

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMDC32-Kar2-CFP-HDEL

Plasmid

#96989

Purpose

Express endoplasmic reticulum (ER) marker in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

Kar2-CFP-HDEL

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-1

Plasmid

#164858

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-1

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMC-6-ZmBbm-9

Plasmid

#197748

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmBbm

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-C1

Plasmid

#31926

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pMC-1-StUbi-6

Plasmid

#197710

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

StUbi promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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