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pRAN357
Plasmid#120898PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target proteinDepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir451-CMV2-mir1~144
Plasmid#164665PurposeExpress miR-451 and miR-1-1~144 in mammalian cellsDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
EHMT1_exon 3_gRNA
Plasmid#228809PurposeCRISPR targeting of human EHMT1DepositorInsertgRNA targeting EHMT1 exon 3 (EHMT1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP_EHMT1B1-3
Plasmid#228811PurposeExpression of mouse EHMT1 with deletion of aa 1185-1298DepositorInsertEHMT1 (Ehmt1 Mouse)
UseTagsEGFPExpressionMammalianMutationdeletion of aa 1185-1298PromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP_EHMT1B1-4
Plasmid#228812PurposeExpression of mouse EHMT1 with deletion of aa 809-1298DepositorInsertEHMT1 (Ehmt1 Mouse)
UseTagsEGFPExpressionMammalianMutationdeletion of aa 809-1298, See Depositor CommentsPromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
NL4-3Δ6-drEGFP-IRES-mThy1.2
Plasmid#207881PurposeHIV reporter vector expressing dGFP and mThy1.2 from Env locusDepositorInsertdestabilized EGFP-IRES-mThy1.2
UseLentiviralTagsExpressionMutationPromoter5' lentiviral LTRAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol1 - 14xUAS:CaSR-EGFP
Plasmid#191502PurposeA UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.DepositorInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol1 transgenes…TagsExpressionMutationPromoter14X UASAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-IFT20
Plasmid#218719PurposeExpresses C-terminally EGFP-tagged IFT20 in mammalian cellsDepositorInsertIFT20 (IFT20 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald
Plasmid#214468PurposeLentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationS14D S25D S32D T261DPromoterAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH4316
Plasmid#200171PurposePflp-18 LoxP EBFP (stop) LoxP twk-40(gf) GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40(gf) GFPDepositorInserttwk-40 (twk-40 Nematode)
UseTagsGFPExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJH4018
Plasmid#200043PurposePrig-3 FRT let858 (stop) FRT zif-1 SL2 EBFP unc-54 3' UTR C.elegans AVA and other neurons expression of zif EBFPDepositorInsertzif-1 (zif-1 Nematode)
UseTagsEBFPExpressionWormMutationPromoterPrig-3Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
SGLT1(GFP)-MAP17(nb)
Plasmid#216210Purposefor expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam systemDepositorUseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SGLT2(GFP)-MAP17(nb)
Plasmid#216211Purposefor expression of human GFP-tagged SGLT2-MAP17 nanobody complex in BacMam systemDepositorUseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry
Plasmid#214471PurposeLentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationS14D S25D S32D T261DPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]
Plasmid#198381PurposeExpression of SYPB::CRY2olig(535) in neurons of Danio rerioDepositorInsertUAS:sypb-egfp-cry2
UseTol2TagsEGFPExpressionMutationD387APromoterUASAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnCas9-miniSdd3-PBE
Plasmid#204857PurposeFor cytosine base editing using miniSdd3 in monocotyledon plants (transient transformation)DepositorInsertminiSdd3-nSpCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterZmUbi-1Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TALE-R-JAK2-Ddd9-C
Plasmid#204854PurposeFor DdCBE editing using Ddd9 at JAK2 site in human cellsDepositorInsertDdd9-C
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TALE-L-JAK2-Ddd9-N
Plasmid#204853PurposeFor DdCBE editing using Ddd9 at JAK2 site in human cellsDepositorInsertDdd9-N
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-GFP-SPOP (R354H)
Plasmid#197010PurposeMammalian inducible expression vector for GFP-tagged SPOPDepositorInsertSPOP (Spop Mouse)
UseTagsGFPExpressionMammalianMutationR354HPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only
