Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 2,009 results for: D10

Clear keywords
Showing: 801 - 820 of 2009 results

  1. pX330-Flag-dSpCas9-HF1

    Plasmid
    #92115
    Purpose
    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive SpCas9-HF1 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, Q926A
    Promoter
    Cbh
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92115-plasmid-map-sequence-id-197505

    Enlarge

  3. pME464

    Plasmid
    #238068
    Purpose
    Expressed ME-ABE ABE7.10-HRHSK with SpRYCas9
    Depositor
    Alexis Komor
    Article
    Evanoff et al Nat Biotechnol. 2026 Mar 18. doi: 10.103
    Insert
    TadA*7.10(L36H/L51R/Y123H/C146S/N157K)-m_SpRYnCas9(D10A)
    Use
    CRISPR
    Tags
    SV40 bpNLS and SV40 bpNLS-P2A-mCherry
    Expression
    Mammalian
    Mutation
    TadA modifications include L36H, L51R, Y123H, C14…
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    238068-plasmid-map-sequence-id-472664

    Enlarge

  4. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

    Enlarge

  5. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid
    #66708
    Purpose
    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
    Depositor
    Charles Gersbach
    Insert
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Mutation
    D10A and H840A
    Promoter
    hUbC
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66708-plasmid-map-sequence-id-449286

    Enlarge

  11. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

    Plasmid
    #197508
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…
    Promoter
    CMV and T7
    Available Since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197508-plasmid-map-sequence-id-387292

    Enlarge

  17. pHis8-p100(1-899)

    Plasmid
    #44735
    Depositor
    Gourisankar Ghosh
    Insert
    p100 (NFKB2 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    AA 1-899
    Available Since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 801 - 820 of 2009 results