We narrowed to 23,988 results for: Nes

1. pLV-Syn1-mCherry

   Plasmid

   #222967

   Purpose

   Make lentivirus to express mCherry in syn1 expressing cells

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   synapsin I

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_SPSB1_SPRY

   Plasmid

   #178487

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   SPSB1_SPRY (SPSB1 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM33_YES1_SH3

   Plasmid

   #178492

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   YES1_SH3 (YES1 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TREtight-H2b-emiRFP670-P2A-TEVP

   Plasmid

   #174379

   Purpose

   AAV virus for expressing TEVP and H2b-emiRFP670

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   TEVP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRV∆G-NPEST-4Cre

   Plasmid

   #174380

   Purpose

   Rabies vector for expressing Cre recombinase and N gene with PEST domain

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   Cre recombinase
   

   Use

   Rabies

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOT_7-lenti-EFS-SS1-BBz-2A-puro

   Plasmid

   #217990

   Purpose

   Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Xue et al bioRxiv 2024.11.27.625752

   Insert

   SS1-BBz CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiSAMv2

   Plasmid

   #75112

   Purpose

   lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #138149

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138150

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   DR1 A>T

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM33_PDCD6IP_ALIX V-domain

    Plasmid

    #178483

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    PDCD6IP_ALIX V-domain (PDCD6IP Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pN1-GCaMP6m-XC

    Plasmid

    #111543

    Purpose

    Improved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-X

    Depositor

    Xiaodong Liu

    Article

    Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

    Insert

    GCaMP6m-XC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-myc-Atg7

    Plasmid

    #24921

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    Atg7 (ATG7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-CAG-FLEX-BFP2-(mCherry)’

    Plasmid

    #115234

    Purpose

    Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    mTagBFP2 and mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2xFLAGhYAP1

    Plasmid

    #17791

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138145

    Purpose

    Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-myc-Atg5

    Plasmid

    #24922

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    Atg5 (ATG5 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. VPR_dCas9

    Plasmid

    #134601

    Purpose

    Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

    Depositor

    Lasse Ebdrup Pedersen

    Article

    Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

    Insert

    VPR-dCas9-2A-ZsGreen-DR
    

    Use

    Tags

    VPR and ZsGreen1-DR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid

    #223175

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    DR1 A>T

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #223173

    Purpose

    Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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