We narrowed to 2,518 results for: PLS

1. U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE

   Plasmid

   #101852

   Purpose

   Direct reprogramming of adult fibroblasts to neurons

   Depositor

   Malin Parmar

   Article

   Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

   Insert

   Ascl1, Brn2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6, PGK

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-PR rep/cap

   Plasmid

   #197565

   Purpose

   encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

   Depositor

   Casey Maguire

   Article

   Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

   Inserts

   AAV Rep genes
   AAV9 VP1 Cap gene with PR insert
   

   Use

   AAV

   Tags

   Expression

   Mutation

   contains 7mer insert PRPPSTH between amino acids …

   Promoter

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pelB-MBP-HtrA-ss

   Plasmid

   #137066

   Purpose

   E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature HtrA: lacks the HtrA signal peptide

   Promoter

   T7lac

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-IKKbeta (S177E,S181E)-pcw107

   Plasmid

   #64609

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   IKBKB
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   S177E, S181E

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pBT2-4xUAS:P2A-EGFP

   Plasmid

   #127591

   Purpose

   This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides.

   Depositor

   Longhou Fang

   Article

   Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-CMV-dnMCAK

   Plasmid

   #205993

   Purpose

   Over-expression of GFP-dnMCAK

   Depositor

   Floris Foijer

   Article

   Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

   Insert

   dnMCAK
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-Mkk7-JNK2 fusion-pcw107

   Plasmid

   #64618

   Purpose

   Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   Map2k7 linker MAPK9
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   mouse-human fusion

   Promoter

   PGK

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pF CAG luc hygro

   Plasmid

   #67502

   Purpose

   Constitutive expression of firefly luciferase in mammalian cells

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   Firefly luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-PC-GCaMP6f

   Plasmid

   #73503

   Purpose

   GCaMP6f knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   GCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-Rheb (Q64L)-pcw107

    Plasmid

    #64607

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RHEB (RHEB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Q64L

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLVX-dnMCAK

    Plasmid

    #205995

    Purpose

    inducible expression of HA-dnMCAK

    Depositor

    Floris Foijer

    Article

    Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

    Insert

    dnMCAK
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pTight

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-REST_sh_seq1_WPRE

    Plasmid

    #127573

    Purpose

    shRNA to knock down RE1-silencing transcription factor (REST)

    Depositor

    Malin Parmar

    Article

    Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

    Insert

    shRNA for knock down of REST (REST Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF CAG luc puro

    Plasmid

    #67501

    Purpose

    Constitutive expression of firefly luciferase in mammalian cells

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EFIa-Id1-PGK-puro

    Plasmid

    #107735

    Purpose

    Expresses Id1 in mammalian cells

    Depositor

    Alexandre Colas

    Article

    Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

    Insert

    Id1 (T Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1REST_sh_seq2_WPRE

    Plasmid

    #127574

    Purpose

    shRNA to knock down RE1-silencing transcription factor (REST)

    Depositor

    Malin Parmar

    Article

    Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

    Insert

    shRNA for knock down of REST (REST Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBT2-4xUAS:P2A-mCerulean3

    Plasmid

    #127592

    Purpose

    This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

    Depositor

    Longhou Fang

    Article

    Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRetroX GFP Mps1 wt

    Plasmid

    #63702

    Purpose

    Inducible expression of GFP-Mps1 fusion in mammalian cells

    Depositor

    Floris Foijer

    Article

    Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    Mps1 (TTK Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pelB-MBP-BB0238-ss

    Plasmid

    #137046

    Purpose

    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature BB0238: lacks the BB0238 signal peptide

    Promoter

    T7lac

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. myr-FLAG-PIK3CA-pcw107

    Plasmid

    #64605

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    PIK3CA (PIK3CA Bovine)
    

    Use

    Lentiviral

    Tags

    FLAG and myristoylated

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

20. JWW-2 human chimeric monoclonal antibody

    Plasmid

    #66749

    Purpose

    Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

    Depositor

    Richard Roden

    Article

    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

    Inserts

    JWW-2 Heavy Chain
    JWW-2 Light Chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 and rEF1

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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