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Plasmid#222545PurposePiggyBac transposon plasmid for doxycycline inducible expression of RFX5DepositorInsertRFX5 (RFX5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
p007ampGFP
Plasmid#58535PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-SYMPK
Plasmid#222556PurposePiggyBac transposon plasmid for doxycycline inducible expression of SYMPKDepositorInsertSYMPK (SYMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT21
Plasmid#204309PurposeFor generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthpartsDepositorInsertie1-GFPn
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT25
Plasmid#204310PurposeFor generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.DepositorInsertie1-GFPc
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB222
Plasmid#170012PurposeEncodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
LL-37
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-ZCWPW1
Plasmid#222543PurposePiggyBac transposon plasmid for doxycycline inducible expression of ZCWPW1DepositorInsertZCWPW1 (ZCWPW1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-ADRA2A
Plasmid#190753PurposeExpression of ADRA2A with mEGFP tag on N-terminus.DepositorInsertADRA2A (ADRA2A Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJYDC3
Plasmid#162460PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA designed ALFA nanobody, HA tag, HDEL sequ…ExpressionMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
hTMEM115-TEV-3xHA-P2A-mScarlet HDR template
Plasmid#214998PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template
Plasmid#214999PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1-GeneTRAP
Plasmid#218184PurposeTo KO and genetrap mouse Hevin simultaneouslyDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GEARBOCS-SPARCL1 KO
Plasmid#218182PurposeTo KO Hevin in astrocytesDepositorInsertsgRNA (Sparcl1 Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM005
Plasmid#170009PurposeEncodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PvirKAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKB230
Plasmid#170017PurposeEncodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Protegrin-1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3
Plasmid#162456PurposeYeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Vector 1174D
Plasmid#200012PurposeFluorescent based sex separator in Aedes aegypti (SEPARATOR)DepositorInsertEngineered dobulesex splicing module
UseSynthetic BiologyTagsExpressionInsectMutationEngineered dobulesex splicing modulePromoterHr5IE1Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_Gag-EIF4E
Plasmid#212657PurposepiggyBac vector to express an MLV Gag-EIF4E fusion proteinDepositorInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UsePiggybacTagsEIF4EExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cre-MS2_UbC-GFP (Cre_GFP)
Plasmid#219536Purposea. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotorDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSCBE3
Plasmid#133966PurposeExpresses BE3 CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertBE3
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only