We narrowed to 5,837 results for: dna plasmids

1. MvChR1_EYFP_pcDNA3.1

   Plasmid

   #159119

   Purpose

   This plasmid encodes MvChR1 rhodopsin domain

   Depositor

   John Spudich

   Article

   Govorunova et al mBio. 2011 Jun 21;2(3):e00115-11. doi: 1

   Insert

   MvChR1
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.3_SARS2_XBB

   Plasmid

   #194494

   Purpose

   expressing SARS-CoV-2 XBB spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

   Insert

   Spike (XBB) (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

   Promoter

   CMV

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA4 CoV-2 nsp1 3xFLAG

   Plasmid

   #176057

   Purpose

   Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA4TO-3XFLAG Halo WT nsp1

   Plasmid

   #175412

   Purpose

   Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 nsp1 (ORF1ab CoV2)
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

   Plasmid

   #157716

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

   Plasmid

   #157691

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

   Plasmid

   #157699

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.1-SARS-CoV-2-S-ectodomain

   Plasmid

   #235984

   Purpose

   Internally tagged SARS-CoV-2 spike ecto-domain

   Depositor

   Syed Saif Hasan

   Article

   Singh et al J Struct Biol X. 2025 Feb 11;11:100123.

   Insert

   Spike protein (S SARS-CoV-2)
   

   Tags

   8xHis and Twin-Strep tag and internal Twin-Strep …

   Expression

   Mammalian

   Mutation

   aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA 3.1-NbON10-NanoLuc

   Plasmid

   #198691

   Purpose

   Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

   Depositor

   Gualberto González Sapienza

   Article

   Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

   Insert

   anti-SARS-CoV-2 Nucleocapsid nanobody ON10
   

   Tags

   6xHis tag, IgK leader sequence, NanoLuc, and twin…

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-α-COPI-WD40-Arg57Ala

    Plasmid

    #180759

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Arg57Ala
    

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Arg57Ala, Leu1…

    Promoter

    CMV

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-α-COPI-WD40-Tyr139Ala

    Plasmid

    #180761

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Tyr139Ala
    

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Tyr139Ala, Leu…

    Promoter

    CMV

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-α-COPI-WD40-Asp115Ala

    Plasmid

    #180760

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Asp115Ala
    

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Asp115Ala, Leu…

    Promoter

    CMV

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-FRT-TO-Nsp8-Nsp7_GC3opt

    Plasmid

    #157700

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pELF2.1.0-gDNA

    Plasmid

    #132454

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ELF2 (ELF2 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBRCA2.1.0-gDNA

    Plasmid

    #132448

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    BRCA2 (BRCA2 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLF6.1.0-gDNA

    Plasmid

    #132435

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    KLF6 (KLF6 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.3_CoV2_B.1.1.7

    Plasmid

    #170451

    Purpose

    Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Yuan et al Science. 2021 Aug 13;373(6556):818-823.

    Insert

    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
    

    Tags

    NA

    Expression

    Mammalian

    Mutation

    HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

    Promoter

    CMV

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 SARS-CoV-2 N

    Plasmid

    #158079

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 nucleocapsid (N )
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 SARS-CoV-2 E

    Plasmid

    #158080

    Purpose

    Expresses SARS-CoV-2 envelope protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 envelope (E )
    

    Expression

    Mammalian

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 SARS-CoV-2 M

    Plasmid

    #158078

    Purpose

    Expresses SARS-CoV-2 membrane protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 matrix (M )
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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