We narrowed to 1,517 results for: pet-28a

1. pET28:LanIV-Chondromyces

   Plasmid

   #225078

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIV-Chondromyces)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28:LanI-101

   Plasmid

   #225065

   Purpose

   Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanI-101)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28 mePOU-POU

   Plasmid

   #206392

   Purpose

   Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   ePOU
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   POU DNA binding domain only

   Promoter

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-p53 (1-73) (K24N)

   Plasmid

   #62079

   Purpose

   Expression of human p53 (1-73) (K24N) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; K24N

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET28-p53 (1-90) (72P)

   Plasmid

   #62080

   Purpose

   Expression of human p53 (1-90) (72P) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD/PP residues 1-90; 72P

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pSAM

   Plasmid

   #45174

   Depositor

   Jumi Shin

   Article

   Sathiamoorthy et al PLoS One. 2012;7(10):e47259. doi: 10.137

   Type

   Empty backbone

   Use

   Tags

   6xHis and 6xHis/Thrombin/T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pET28-Flag-RBM24-95~236

   Plasmid

   #208634

   Purpose

   for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236

   Depositor

   Quanfu Ma

   Insert

   human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   only aa 95~236, other residues not included

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Brg1 Del4 pET28C

   Plasmid

   #122118

   Purpose

   Expression Brg1 amino acids 1446-1647 in bacteria

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   Brg1 (SMARCA4 Human)
   

   Use

   Tags

   His-T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28 mOct4-POU

   Plasmid

   #206393

   Purpose

   Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   POU DNA binding domain only

   Promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-GFP-mHP1alpha

    Plasmid

    #181912

    Purpose

    Bacterial protein expression vector for GFP-tagged HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28_mouse-Caspase-1-H340D_enzymatic-domain

    Plasmid

    #183388

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (AA130-404)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    H340D

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-p53 (Guinea Pig 1-88)

    Plasmid

    #62059

    Purpose

    Expression of Guinea Pig p53 (residues 1-88) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-88

    Promoter

    T7

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET28_mouse-Caspase-1_enzymatic-domain

    Plasmid

    #183385

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (AA130-404) (Casp1 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28_human-Caspase-4_enzymatic-domain

    Plasmid

    #183381

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (AA94-377) (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28_mouse-Caspase-11_enzymatic-domain

    Plasmid

    #183382

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-11 (AA100-373) (Casp4 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28_canine-Caspase-1/4_enzymatic-domain

    Plasmid

    #183383

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-Dnmt3a3L-sc27

    Plasmid

    #71827

    Purpose

    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

    Depositor

    Albert Jeltsch

    Article

    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

    Insert

    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-AviTag-DogCatcher-MBP

    Plasmid

    #171928

    Purpose

    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogCatcher-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    DogCatcher added to N-terminus of MBP via a GSGES…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28-sfGFP-DogTag Loop A

    Plasmid

    #171779

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Val2…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21b-Caspase-3(D3A,V266E)

    Plasmid

    #90091

    Purpose

    Expresses caspase D3A and V266E mutant in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A V266E

    Promoter

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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