We narrowed to 919 results for: tac promoter

1. pYTRW18K_3T5

   Plasmid

   #177288

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR and mCherry

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   mCherry
   

   Use

   Synthetic Biology; Yeast expression, tn5 genomic …

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC50

   Plasmid

   #104824

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Insert

   Glyma.08g081600, Glyma.05g126600
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFSynW Syt9 IRES mRuby3

   Plasmid

   #195700

   Purpose

   Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   encodes only the first 68 amino acids of Tac1

   Promoter

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. gRNAs[bTub+Tra].1046D

   Plasmid

   #112693

   Purpose

   express two gRNA targeting bTub & Tra under dU6-3 promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   U6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN279

   Plasmid

   #91654

   Purpose

   Express sgRNA targeting human PTN

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   PTN (PTN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. YjdC-R82A_pET-14b

   Plasmid

   #233276

   Purpose

   Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

   Depositor

   Mohan Babu, Andrew Emili

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   YjdC (yjdC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 82…

   Promoter

   T7 promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. YjdC-R64A_pET-14b

   Plasmid

   #233263

   Purpose

   Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

   Depositor

   Mohan Babu, Andrew Emili

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   YjdC (yjdC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 64…

   Promoter

   T7 promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPlacPbuCas13b-gRNA-T-deGFP

   Plasmid

   #184840

   Purpose

   Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 and lac promoter

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPlacPbuCas13b-gRNA-T-MS2

   Plasmid

   #184839

   Purpose

   Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 and lac promoter

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. EC71167

    Plasmid

    #154066

    Purpose

    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Promoter

    Zea mays Ubiquitin Promoter

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPN014

    Plasmid

    #91588

    Purpose

    Express sgRNA targeting human CSMD1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CSMD1 (CSMD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid

    #232116

    Purpose

    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    BCKDK (BCKDK Human)
    

    Use

    Tags

    6 x His, Biotin acceptor peptide (BAP), Maltose-B…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLC-CUL4A K705R-P2A-Puro

    Plasmid

    #124305

    Purpose

    Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Lysine 705 to Arginine

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172670

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    SDHB pegRNA and SDHB_nick-sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172669

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    RNF2 pegRNA and RNF2_nick-sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKD072

    Plasmid

    #136472

    Purpose

    SSB-C-term-GFP fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-C-term-GFP (ssb E. coli)
    

    Use

    Tags

    superfolder GFP

    Expression

    Bacterial

    Mutation

    superfolder GFP attached to C-terminus at Phe 178

    Promoter

    T7 promoter

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pk335-CARGO-6mer-Fgf5Pro

    Plasmid

    #227479

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to Fgf5 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pk335-CARGO-5mer-Fgf5Pro

    Plasmid

    #227480

    Purpose

    5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to Fgf5 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pk335-CARGO-4mer-Fgf5Pro

    Plasmid

    #227481

    Purpose

    4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to Fgf5 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCL.176

    Plasmid

    #184997

    Purpose

    Express -Eco1 RNF2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    RNF2 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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