We narrowed to 26,768 results for: VIT

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pRSETa-Amrose v1

Plasmid

#79537

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 1

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-EKAR

Plasmid

#181858

Purpose

Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-EKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-146a-Decoy.hPGK.GFP.WPRE

Plasmid

#46590

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-146a Decoy (MIR146A, Mir146 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pJ207 Delta-cpc-KanR

Plasmid

#51468

Purpose

Deletes the cpc operon in Synechocystis and confers kanamycin resistance

Depositor

Anastasios Melis

Article

Kirst et al Biochim Biophys Acta. 2014 Oct;1837(10):

Insert

Replacing the cpc operon with kanamycin resistance

Use

Synthetic Biology

Promoter

Synechocystis endogenous cpc-operon promoter

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

Plasmid

#159658

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

Plasmid

#159657

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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vsv-CenpB 1-166-Sgo1 N61I-GFP

Plasmid

#108499

Purpose

expression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1, no PP2A binding N…

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pVLAD6-mmPtch1-B-SBP

Plasmid

#120886

Purpose

BacMam vector for Ptch1-B expression

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

SBP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(K14A)

Plasmid

#86562

Purpose

Expresses human full-length Akt1(K14A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

K14A mutation in the PH domain

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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hs-lambda btl

Plasmid

#17581

Depositor

Denise Montell

Article

Lee et al Genes Dev. 1996 Nov 15. 10(22):2912-21.

Insert

lambda-btl (btl Fly)

Expression

Insect

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pBEG R2-eCFP-L3 #BGV202

Plasmid

#48977

Purpose

Supplies the selection marker (eCFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Insert

eCFP

Use

Gateway cloning vector

Tags

IRES (strong)

Promoter

NA

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2puro-Gnaq

Plasmid

#170302

Purpose

A knock-out vector for the mouse Gnaq

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Gnaq gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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LZRSpBMN-Z‐GFP‐Eps8

Plasmid

#74935

Purpose

Expresses mouse Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

Insert

Eps8 (Eps8 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
LZRS ATG12 ires zeo

Plasmid

#67531

Purpose

For retroviral based stable expression of human ATG12

Depositor

Stephen Tait

Article

Haller et al Autophagy. 2014;10(12):2269-78. doi: 10.

Insert

APG12 (ATG12 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pFastBac-His6-Mps1-KD

Plasmid

#114064

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

Insert

Mps1-KD (TTK Human)

Tags

His6

Expression

Insect

Mutation

Mps1 KD (D664A)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgHAL

Plasmid

#102315

Purpose

genetic depletion of HAL

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgHAL (HAL Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

Plasmid

#160635

Purpose

TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

Insert

AcrIIA4

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPK1E-1-gfp

Plasmid

#48133

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-NNQ-HA-GFP

Plasmid

#121164

Purpose

Expresses mmPtch1-B NNQ variant

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, N…

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP

Plasmid

#120909

Purpose

Expresses Ptch1-B (I766FV111FL114F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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