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pUASTattBVhhGFP4-Cry2

Plasmid

#244065

Purpose

antiGFP-nanobody fused to the light sensitive dimerization domain CRY2

Depositor

Markus Affolter

Publication

Bao et al G3 (Bethesda). 2025 Jun 4;15(6):jkaf073.

Insert

VhhGFP4-Cry2-myc

Tags

myc

Expression

Insect

Available since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pUASTattBRhokinase-CIBN

Plasmid

#244066

Purpose

active kinase domain of Rok fused to the light sensitive dimerization domain CIBN

Depositor

Markus Affolter

Publication

Bao et al G3 (Bethesda). 2025 Jun 4;15(6):jkaf073.

Insert

Rhokinase-CIBN-HA

Tags

HA

Expression

Insect

Available since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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yEPAC-R279L pDRF1-GW

Plasmid

#246454

Purpose

Expresses the non-responsive yEPAC-R279L sensor in budding yeast.

Depositor

Bas Teusink

Publication

Botman et al Mol Biol Cell. 2021 Jun 15;32(13):1229-1

Insert

yEPAC-R269L

Expression

Yeast

Promoter

PMA1

Available since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR2-3A

Plasmid

#245789

Purpose

Expression of mutated bovine arrestin-2 (I386A, V387A, F388A) in bacteria

Depositor

Vsevolod Gurevich

Publication

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-2 (ARRB2 Bovine)

Expression

Bacterial

Mutation

I386A, V387A, F388A

Promoter

lac

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-TrF

Plasmid

#245788

Purpose

Expression of mutated bovine arrestin-3 (L394Stop) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Publication

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

L394Stop

Promoter

CMV

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-REF

Plasmid

#245786

Purpose

Expression of mutated bovine arrestin-3 (R170E) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Publication

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

R170E

Promoter

CMV

Available since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3F

Plasmid

#245783

Purpose

Expression of bovine arrestin-3 tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Publication

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β1β6-EGFP

Plasmid

#242826

Purpose

Encodes human integrin β1 chimera containing integrin β6 cytoplasmic domain and C-terminal EGFP tag

Depositor

Cheng-han Yu

Publication

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 1, transcript variant 1A (ITGB1 Human)

Expression

Mammalian

Mutation

Integrin β1 chimera containing the cytoplasmic ta…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDS_TempRecording

Plasmid

#241789

Purpose

pBBR1MCS replicative vector for Cas1-Cas2-RT and Spacer for transcriptional recording with a temperature sensor

Depositor

Marc Güell

Publication

Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

Insert

FBFP, Cas1-Cas2-RT_and CRISPR Array

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β6β1-EGFP

Plasmid

#242829

Purpose

Encodes human integrin β6 chimera containing integrin β1 cytoplasmic domain and C-terminal EGFP tag

Depositor

Cheng-han Yu

Publication

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 6, transcript variant 1 (ITGB6 Human)

Expression

Mammalian

Mutation

Integrin β6 chimera containing the cytoplasmic ta…

Available since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mNeonGreen

Plasmid

#231288

Purpose

For transiently expressing fusion proteins with N terminally tagged mNeonGreen in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mNeonGreen

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-Renilla

Plasmid

#231291

Purpose

For transiently expressing fusion proteins with N terminally tagged Renilla luciferase in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

Renilla

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-StrepIII

Plasmid

#231292

Purpose

For transiently expressing fusion proteins with N terminal StrepIII tags in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

StrepIII

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-mNeonGreen

Plasmid

#231296

Purpose

For transiently expressing fusion proteins with C terminally tagged mNeonGreen in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mNeonGreen

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-Renilla

Plasmid

#231299

Purpose

For transiently expressing fusion proteins with C terminally tagged Renilla luciferase in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

Renilla

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-RPP25-FLAG (pAVA3895)

Plasmid

#239236

Purpose

Expresses C-FLAG tagged Rpp25 (CMV-RPP25-FLAG)

Depositor

Andrei Alexandrov

Publication

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

RPP25-FLAG

Tags

FLAG

Expression

Mammalian

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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F-IKKε v2

Plasmid

#169773

Purpose

Express IKKε v2 in mammalian cells

Depositor

Sung-Liang Yu

Publication

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2

Tags

Flag tag

Expression

Bacterial and Mammalian

Available since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pART15-C1

Plasmid

#127395

Purpose

Expresses amcyan fluorescent gene

Depositor

Ryan Senger

Publication

Tanniche et al Biotechnol Prog. 2023 May-Jun;39(3):e332

Insert

AmCyan

Expression

Bacterial

Promoter

PBAD

Available since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEP17

Plasmid

#229234

Purpose

PutP (Sodium/proline symporter) under tet operator, TetR

Depositor

Yolanda Schaerli

Publication

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Insert

putP (Sodium/proline symporter) (putP E. coli)

Expression

Bacterial

Promoter

tet operator

Available since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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POC1358

Plasmid

#236103

Purpose

Assembled plasmid containing four inserts (from POC1343, POC1344, POC1345 and POC1346) assembled in POC1355

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Expression

Bacterial

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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