We narrowed to 22,799 results for: promoter

1. pAAV-phSyn-flex-SypHaloTag

   Plasmid

   #172280

   Purpose

   Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.

   Depositor

   Danny Winder

   Insert

   synaptophysin-HaloTag
   

   Use

   AAV and Cre/Lox

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   rat synapsin

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

   Plasmid

   #219689

   Purpose

   Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PsB (mtagBFP2, mCherry, NeonGreen)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

   Plasmid

   #217970

   Purpose

   Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

   Depositor

   Robert Piper

   Insert

   2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MUT_S1-N

   Plasmid

   #218398

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-responsive promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MUT_N-H1

   Plasmid

   #218397

   Purpose

   3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-responsive promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-SYN-eGFP-oPRE

   Plasmid

   #218681

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

   Depositor

   Bart Nieuwenhuis

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR6K-GFP-CASTIF

   Plasmid

   #199653

   Purpose

   R6K plasmid encoding a type I-F CAST but no CRISPR array

   Depositor

   IIya Finkelstein

   Insert

   CAST I-F systems
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCT03

   Plasmid

   #212150

   Purpose

   Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.

   Depositor

   Kang Zhou

   Insert

   alanine dehydrogenase
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   PT7

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEV-G2-Km ymTurquoise2

   Plasmid

   #193958

   Purpose

   TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeast

   Depositor

   Bas Teusink

   Insert

   ymTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p416-GPD-HA-PPK(mutant)

    Plasmid

    #183944

    Purpose

    Expresses HA-tagged catalytic mutant of E. coli PPK from yeast GPD promoter

    Depositor

    Michael Downey

    Insert

    PPK(mutant) (ppk E. coli)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    mutations in His435, His454, and His592 to Alanine

    Promoter

    GPD

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid

    #177208

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL1271

    Plasmid

    #129282

    Purpose

    Expression plasmid with pLL1270 as backbone and alpha-Xylp(Ga0256695_ 1211)

    Depositor

    Lee Lynd

    Insert

    alpha-Xylp_1211 (Ga0256695_ 1211)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. brr2-R1107M pRS313

    Plasmid

    #111417

    Purpose

    brr2-R1107M (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R1107M aa mutation (note, not an RP allele)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF4Ag NanoLuc (ATG-42)

    Plasmid

    #137777

    Purpose

    Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

    Depositor

    Lance Encell

    Article

    Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

    Insert

    NanoLuc (Nluc)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    NanoLuc contains 16 amino acid changes compared t…

    Promoter

    T7 and CMV

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. ppyCAG_RNaseH1_WKKD

    Plasmid

    #111905

    Purpose

    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

    Promoter

    Available since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid

    #171379

    Purpose

    Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized Cas13X.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pInducer10b-EGFP-KRAS G12V

    Plasmid

    #164925

    Purpose

    Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

    Inserts

    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glycine 12 changed to valine (G->T 3156)

    Promoter

    Ubc Promoter and Ubc promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRE-8XGTIIC-DsRED-DD

    Plasmid

    #115798

    Purpose

    lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP responsive promoter (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    DsRED-DHFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-HA-NONO

    Plasmid

    #127655

    Purpose

    Over-express HA-tagged human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO (NONO Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-SMARCA4

    Plasmid

    #65391

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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