We narrowed to 24,448 results for: promoter
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Plasmid#208967PurposeUnfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)
Plasmid#208944PurposeA control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationdSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCas9-sgCh2-2-Blast
Plasmid#199645PurposeExpresses Cas9 and sgRNA control guide targeting intergenic regionDepositorInsertN/A
UseLentiviralAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-K525E
Plasmid#91985PurposeExpresses FLAG-HA-AGO2 (K525E)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
px330-CIB1-dCas9
Plasmid#63665PurposeMammalian gRNA expression vector also expressing CIB1-dCas9DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6 and CMVAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 KBTBD4 P311PP
Plasmid#184628PurposeMammalian expression vector with N-terminal 3xFlag for KBTBD4 P311PP expression in mammalian cellsDepositorInsertN-terminal 3xFlag tagged KBTBD4 P311PP (KBTBD4 Human)
ExpressionMammalianMutationP311PPPromoterCMVAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 KBTBD4 R312RG
Plasmid#184630PurposeMammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cellsDepositorInsertN-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)
ExpressionMammalianMutationR312RGPromoterCMVAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_LN-HA-AGO2-WT
Plasmid#92009PurposeExpresses Lambda-N-HA-AGO2 (WT)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mGSDMD1-251
Plasmid#111565PurposeExpress ELANE-cleaved mouse GSDMD in mammalian cellsDepositorAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MigR1-AE9aP
Plasmid#12434DepositorUseRetroviralTagsHAExpressionMammalianMutationAML1-ETO9a cDNA as in t(8;21) patientsAvailable SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)
Plasmid#208969PurposeRecruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertEcKlenow-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationEcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-ACSL4-FL
Plasmid#202515PurposeExpressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)DepositorAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human FLAG-WDR20
Plasmid#217618PurposeMammalian expression of FLAG tagged WDR20DepositorInsertWDR20 (WDR20 Human)
ExpressionMammalianAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)
Plasmid#208955PurposeA variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-PCAF (delta5-53a.a.)
Plasmid#65388PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertPCAF (KAT2B Human)
TagsEmGFP and V5ExpressionMammalianMutationlost 13-159bp of the ORFPromoterCMVAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
HEpl017
Plasmid#179992PurposepCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoterDepositorInsertTadA8e-dSiCas12i(D1049A)
ExpressionMammalianAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS
Plasmid#209781PurposeExpresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoterDepositorInsertcTNT
UseAAVTagsHA and OLLASPromotercTNTAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS
Plasmid#209782PurposeExpresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoterDepositorInsertsgRNA
UseAAVTagsHA and OLLASPromotercTNTAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEX301-ARF6-T157N-TagRFP
Plasmid#162030PurposeFast cycling ARF mutantDepositorAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A/N847Q-pBabe-Puro
Plasmid#25948DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…Available SinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only