161,799 results

1. pWN_U6-B2M-miniGag

   Plasmid

   #228957

   Purpose

   miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_PAsfCherry2(1-10)

   Plasmid

   #82612

   Purpose

   Express PAsfCherry2(1-10) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   PAsfCherry2(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Glu 118 to Gln, changed Thr 128 to Ile

   Promoter

   CMV

   Available since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi-RB

   Plasmid

   #60522

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. GLP1R-Tango

   Plasmid

   #66295

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GLP1R (GLP1R Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-TagRFP-T_A1aY1

   Plasmid

   #158754

   Purpose

   Expression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

   Insert

   6xHistidine_TagRFP-T_A1aY1
   

   Use

   Tags

   6x-Histidine tag and TagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLM-mCerulean-cMyc

   Plasmid

   #23244

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

   Insert

   mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Zeo-dTAG

   Plasmid

   #207705

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

   Plasmid

   #188899

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSP3535

   Plasmid

   #46886

   Purpose

   Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

   Depositor

   Gary Dunny

   Article

   Bryan et al Plasmid. 2000 Sep;44(2):183-90.

   Type

   Empty backbone

   Use

   Nisin-inducible expression; gram-positive bacteri…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PnisA

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-mKelly2

    Plasmid

    #119783

    Purpose

    Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mKelly2
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG_Xph20-mRuby2-CCR5TC

    Plasmid

    #135531

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICE-HA-NLS-I-PpoI

    Plasmid

    #46963

    Purpose

    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    NLS-I-PpoI
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TET

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. rEstus

    Plasmid

    #209767

    Purpose

    To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.

    Depositor

    Stefan Heinemann

    Article

    Rühl et al bioRxiv 2023.

    Insert

    rEstus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

    Plasmid

    #60661

    Purpose

    Flp-dependent expression of Kir2.1-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    FLEX- Kir2.1-T2A-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6.2_3xFLAG_V5_ eGFP_V5

    Plasmid

    #160111

    Purpose

    Expresses eGFP in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. rAAV2/cMG

    Plasmid

    #184539

    Purpose

    AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

    Insert

    AAV-cMG capsid
    

    Use

    AAV; Helper vector for packaging

    Tags

    Expression

    Mutation

    changes on AAV9 capsid: K449R, A589D; insertion o…

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

    Plasmid

    #193294

    Purpose

    episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Discosoma, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-HA-USP20

    Plasmid

    #22573

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP20 (USP20 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-mTagBFP2-T2A-iCre (v2)

    Plasmid

    #125822

    Purpose

    Monomeric TagBFP2 and iCre recombinase (version 2)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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