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pSKB3-SaGGR-I206F-L377H (JBEI-7202)

Plasmid

#110152

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-I206F-L377H mutant gene

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-zfh1

Plasmid

#86381

Purpose

Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Insert

D. melanogaster zfh1 enhancer

Use

Stap-seq screening vector

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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Tol2-UAS-Dendra2-miR-223 sponge

Plasmid

#97146

Purpose

For miR-223 knock down

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223 sponge

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgYAP-2

Plasmid

#121423

Purpose

sgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgYAP-2

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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RCAS(A) human p110 alpha E545K

Plasmid

#52211

Purpose

expresses human p110 alpha with E545K mutation in avian cells

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2005 Jan 18;10

Insert

p110 alpha E545K (PIK3CA Human)

Use

Retroviral; Avian

Mutation

Glutamic Acid 545 to Lysine

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_PTK6-1/1

Plasmid

#91441

Purpose

Protein expression and purification of human SH3 domain construct PTK6-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PTK6-1/1 (PTK6 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-mTAZ-WW (43)

Plasmid

#40916

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Contains mTAZ WW domain (43 amino acids, 122-164)

Promoter

Tac

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-STAG2-S653Stop

Plasmid

#31976

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed Serine 653 to Stop codon

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pHH0103_SH3RF1-1/4

Plasmid

#91544

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-1/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-2/4

Plasmid

#91457

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-2/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-2/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-4/4

Plasmid

#91374

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-4/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-4/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF3-2/2

Plasmid

#91356

Purpose

Protein expression and purification of human SH3 domain construct SH3RF3-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF3-2/2 (SH3RF3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-3/4

Plasmid

#91366

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-3/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-3/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF3-1/2

Plasmid

#91280

Purpose

Protein expression and purification of human SH3 domain construct SH3RF3-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF3-1/2 (SH3RF3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR4_FLAG/HA_EWSR1_His6

Plasmid

#26364

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Tags

FLAG/HA and His6

Mutation

delta 657 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pLVTHM-shREV3-4

Plasmid

#38033

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shREV3-4 (Rev3l, Rev3l, REV3L Rat, Mouse, human, Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
TRIN-E-shTAF12.376

Plasmid

#105573

Purpose

Dox-inducible mirE TAF12 shRNA/dsRED expression with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only
pGEX 4T1 hBad(1-101)

Plasmid

#35568

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

bad (BAD Human)

Tags

Flag

Expression

Bacterial

Mutation

residues 1-101

Available Since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ratFDH

Plasmid

#135813

Purpose

Expresses rat FDH (also referred to as AldH1L1) in mammalian cells.

Depositor

Sergey Krupenko

Article

Krupenko et al Cell Growth Differ. 2002 May;13(5):227-3

Insert

Aldh1l1 (Aldh1l1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pIS1 IMP-1 short UTR

Plasmid

#21639

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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