We narrowed to 42,415 results for: INA

1. AAV-mPGK-eGFP (beta-globin intron)

   Plasmid

   #162513

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

   Depositor

   Bart Nieuwenhuis

   Article

   Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mPGK

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORTMAGE-2

   Plasmid

   #72677

   Purpose

   Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.

   Depositor

   Csaba Pál

   Article

   Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

   Insert

   mutL E32K
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   E32K mutation conferring dominant mutator phenoty…

   Promoter

   pL promoter

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-G1-ATP6-Myc-FLAG (puromycin)

   Plasmid

   #86851

   Purpose

   mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

   Depositor

   Matthew O'Connor

   Article

   Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

   Insert

   ATP6 (ATP6 Human)
   

   Use

   Tags

   ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

   Expression

   Mammalian

   Mutation

   codon corrected

   Promoter

   CMV

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AMA1-bio

   Plasmid

   #47741

   Purpose

   Expresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised AMA1
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA AXL

   Plasmid

   #105932

   Purpose

   Mammalian expression of myc-His tagged AXL

   Depositor

   Rosa Marina Melillo

   Article

   Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

   Insert

   AXL (AXL Human)
   

   Use

   Tags

   His and myc

   Expression

   Mammalian

   Mutation

   Promoter

   tet-inducible CMV

   Available Since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAB46 (CK2beta)

   Plasmid

   #27085

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P1-HA-MEK1

   Plasmid

   #53159

   Purpose

   Expresses GST-HA tagged human MEK1 from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-TEX264-deltaLRR-His6

   Plasmid

   #220215

   Purpose

   Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   c.4-75del / p.2-24del

   Promoter

   T7

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE8.17-m

   Plasmid

   #136298

   Purpose

   expresses ABE8.17-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.17-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pARC8-LambdaRedBeta-EcSSB

    Plasmid

    #162572

    Purpose

    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.

    Depositor

    George Church

    Article

    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

    Inserts

    Lambda Red-Beta
    E. coli SSB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    May 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

    Plasmid

    #219548

    Purpose

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The (SAGG)x4 linker between GAL4(1-65) and the PC…

    Promoter

    CMVd1

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid

    #123363

    Purpose

    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    EGFP-NLS
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE

    Plasmid

    #203847

    Purpose

    Flp-dependent expression of Chronos fused to EGFP

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    Chronos
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SID4x-dCas9-KRAB

    Plasmid

    #106399

    Purpose

    Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain

    Depositor

    Jason Gertz

    Article

    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

    Inserts

    SID4x (MXD1 Human)
    KRAB (ZNF10 Human)
    

    Use

    Tags

    FLAG, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAuroraA-GFP-AURKA_K162M-mCherry

    Plasmid

    #99880

    Purpose

    Expression of GFP-AURKA K162M-mCherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP and mCherry

    Expression

    Mammalian

    Mutation

    K162M (kinase-dead)

    Promoter

    AURKA minimal

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-Epac1 CAAX

    Plasmid

    #118312

    Purpose

    Expression of human Epac1

    Depositor

    Philip Stork

    Article

    Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

    Insert

    Epac1 CAAX (RAPGEF3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal 20aa of KiRas

    Promoter

    CMV

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-hAtg12

    Plasmid

    #87869

    Purpose

    Express a EGFP-human Atg12/Apg12 in mammalian cells

    Depositor

    Isei Tanida, Eiki Kominami

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    ATG12 (ATG12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT899

    Plasmid

    #228288

    Purpose

    Constitutive mutant rPhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    rphlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K

    Promoter

    KpGAPDH promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9::BFP-humanized

    Plasmid

    #44247

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 fused to BFP
    

    Use

    CRISPR and Retroviral

    Tags

    2xNLS & tagBFP

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMHE-mH33B-mClover3

    Plasmid

    #171496

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H3.3B (H3f3b Mouse)
    

    Use

    Pgehme

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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