We narrowed to 31,976 results for: LIS

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LentiCRISPR v2 RB1 sgRNA #1

Plasmid

#202516

Purpose

Expressing Cas9 and sgRNA targeting human RB1 gene

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

RB1 sgRNA (RB1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sg_Cdkn1a_i1-ipUSEPR-TR657

Plasmid

#228953

Purpose

Knockdown of Cdkn1a in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Cdkn1a_i1 (Cdkn1a Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Cdkn1a_i2-ipUSEPR-TR657

Plasmid

#228954

Purpose

Knockdown of Cdkn1a in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Cdkn1a_i2 (Cdkn1a Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Trp53_i4-ipUSEPR-TR657

Plasmid

#228930

Purpose

Knockdown of Trp53 in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Trp53_i4 (Trp53 sequence: GTCGCTACCTACAGCCAGGA, Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-CREM

Plasmid

#178442

Purpose

doxycycline-inducible expression of Human CREM in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

CREM (CREM Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-DN (T22N)

Plasmid

#101049

Purpose

Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

T22N

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro hGSDME gRNA1

Plasmid

#223519

Purpose

knocking out hGSDME in human cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

Plasmid

#163991

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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hyABE

Plasmid

#196096

Purpose

Expresses hyABE

Depositor

Xiahui Zhang

Article

Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

Insert

hyABE

Use

CRISPR

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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DTX2

Plasmid

#228447

Purpose

Baculovirus expression for structure determination. May not contain entire coding region of gene.

Depositor

Cheryl Arrowsmith

Article

Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

Insert

DTX2 (DTX2 Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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Chr1q_Cassette-Integration_gRNA

Plasmid

#195126

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-CD79A-YFP

Plasmid

#187004

Purpose

Expression of human CD79A as YFP fusion protein

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

CD79A (CD79A Human)

Use

Lentiviral

Tags

YFP

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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GNAS_pcDNA6.2/EmGFP-Bsd

Plasmid

#176976

Purpose

Mammalian expression vector encoding GNAS and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GNAS (GNAS Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-PRMT6(V86K/D88A)

Plasmid

#164698

Purpose

Expression of PRMT6(V86K/D88A) with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

Insert

PRMT6 (PRMT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

V86K, D88A

Promoter

CMV

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-GFP(1-10)opti

Plasmid

#129416

Purpose

Expressing codon-optimized GFP(1-10) fragment in human cells

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

codon-optimized GFP(1-10)

Use

CRISPR, TALEN, and Unspecified ; Human safe harbo…

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEBB HA CCDC22

Plasmid

#74903

Purpose

Express CCDC22 in mammalian cells

Depositor

Ezra Burstein

Article

Starokadomskyy et al J Clin Invest. 2013 May;123(5):2244-56.

Insert

CCDC22 (CCDC22 Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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AAV_Nanog HMEJ donor

Plasmid

#97321

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Nanog HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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mCardinal-H2B-C-10

Plasmid

#56162

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H2B (H2BC11 Human)

Tags

mCardinal

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
p426_Cas9_gRNA-R-RDS1a

Plasmid

#87405

Purpose

p426_Cas9_gRNA-RDS1a without the ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting RDS1a sequence ATTCAATACGAAATGTGTGC in yeast chromosome 3.

Depositor

Aindrila Mukhopadhyay

Article

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting RDS1a

Use

CRISPR

Promoter

ADH1, pTyrosine

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG 5E

Plasmid

#185341

Purpose

Assessing G2BR function in AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

Flag, 6xHis

Expression

Mammalian

Mutation

R382E/Q383E/K390E/R398E/R400E

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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