We narrowed to 31,746 results for: son
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Plasmid#167502PurposeExpression of dual inducible Cas9 with hALB promoterDepositorInsertCas9
UseCRISPR, Lentiviral, and LuciferaseTagsFLAGExpressionMammalianPromoterhALBAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRM14
Plasmid#163693Purposehsp70 promoter inducible expression of DHB:mNeonGreen-P2A-H2B:mScarletDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseUnspecified; Zebrafish expressionTagsmNeonGreen and mScarletPromoterHSP70Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRM15
Plasmid#163694Purposehsp70 promoter inducible expression of DHB:mScarlet-P2A-H2B:miRFP670DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseZebrafish expressionTagsmScarlet and miRFP670PromoterHSP70Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD2529-CAG-HA-mouse GARP
Plasmid#180258PurposeMouse full length GARP expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGARP (Lrrc32 Mouse)
TagsHAExpressionMammalianAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mNeongreen-Blast
Plasmid#169229PurposeTargeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDouble SapI flanked mNeongreen-P2A-Blast
UseTargeting vector backboneAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LV_EF1a_FLAG-Ikkbeta(S177E,S181E)-P2A-Hygro_Barcode
Plasmid#170226PurposeBarcoded lentiviral vector to express Ikkbeta (S177E, S181E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10
Plasmid#172915PurposeGal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP7f-T2A-tdTomato
Tags6xHis and T2A-tdTomatoExpressionInsectPromoterhsp70Available sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SARS2SΔC-H2gp41
Plasmid#170389PurposeGeneration of SARS-CoV-2 spike pseudotyped lentivirusDepositorInsertSARS-CoV-2 Spike (S SARS-CoV-2)
TagsHIV gp41ExpressionMammalianMutationDeletion of C terminal and fusion with H2 from HI…PromoterCMVAvailable sinceJune 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)
Plasmid#164041PurposeCRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-CRISPR-TagV2
UseCRISPRExpressionMammalianAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ208
Plasmid#162681PurposeLidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertPETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
TagsHisExpressionBacterialMutationReplacement of W185-F191 with G251-T472 from MHET…PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCJ209
Plasmid#162682PurposeLidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
TagsHisExpressionBacterialMutationReplacement of G251-T472 with W185-F191 from PETa…PromoterT7/lacAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV_UdgX-Anc689-UdgX-nCas9-RBMX
Plasmid#163552PurposeMammalian CG-to-GC base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUdgX-Anc689-UdgX-nCas9-RBMX
UseCRISPRExpressionMammalianMutationnCas9 (D10A)Available sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
prk5-MFSD12-TEV-3xFLAG-3xHA
Plasmid#149504PurposeTransient expression of human MFSD12 with recoded nucleotide sequence.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMFSD12 (MFSD12 Human)
UseTransient expressionTagsTEV-3XHA-3XFLAGExpressionMammalianMutationCodon Optimized SequencePromoterCMVAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKS031 - pCAGGS-3XFLAG-BORIS-eGFP-bpA
Plasmid#156443PurposeFor transient expression of mouse BORIS (CTCFL) tagged with eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmouse BORIS (Ctcfl Mouse)
ExpressionMammalianMutationnoneAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc:P4:gs:nls:ABI
Plasmid#135985PurposeExpresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP4 coiled-coil fused to the ABI domain
TagsMycExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE-CR-PINK1-C-TAP
Plasmid#128510PurposeLentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.DepositorInsertPTEN induced kinase 1 (PINK1 Human)
UseLentiviralTagsFLAG and HA tagsExpressionMammalianMutationSynonymous silent mutation in Q5 to render sgRNA …PromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)
Plasmid#117856PurposeThis plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.DepositorPublicationAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx1
Plasmid#116853PurposePI(3,4)P2 biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPLEKHA1 (PLEKHA1 )
TagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationAmino acids 169.329Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY2-mCherry-iTrkA
Plasmid#106168Purposemammalian expression of CRY2PHR (aa 1-498) fused to mCherry and intracellular domain of TrkA (aa 450-799)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRY2PHR mCherry iTrkA (CRY2 Mustard Weed, Rat)
ExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pONSY-CoNMM:mCherry
Plasmid#111878PurposeThis plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCapsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
UseCapsaspora owczarzakiTagsmCherryPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by