166,289 results

1. pcDNA3/Myc-DNMT3A

   Plasmid

   #35521

   Purpose

   Mammalian expression of human DNMT3A with myc tag

   Depositor

   Arthur Riggs

   Article

   Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

   Insert

   DNMT3A (DNMT3A Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-alphasynuclein-A53T

   Plasmid

   #40823

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   A53T (aggregates more rapidly than the wild-type …

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. hPU.1ΔN165-pET28b

   Plasmid

   #199796

   Purpose

   Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

   Depositor

   Gregory Poon

   Article

   Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

   Insert

   spi1 (SPI1 Human)
   

   Tags

   None

   Expression

   Bacterial

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiGuide-Hygro-eGFP

   Plasmid

   #99375

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct

   Plasmid

   #175554

   Purpose

   Targeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   NANOG
   

   Use

   Mouse Targeting

   Tags

   FKBPV-HA2-P2A-eGFP

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR

   Plasmid

   #42875

   Purpose

   A crRNA expression plasmid for targeting a specific sequence.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR-BsaI
   

   Use

   CRISPR; E.coli

   Expression

   Bacterial

   Available Since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNF02-mLychee

   Plasmid

   #219400

   Purpose

   Constitutive expression of mLychee in E. coli

   Depositor

   Christian Lesterlin

   Article

   Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

   Insert

   mLychee
   

   Expression

   Bacterial

   Promoter

   proDp

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMEH365 Hspa8 HA

   Plasmid

   #168219

   Purpose

   Mammalian expression of heat shock 70 kDa protein 8 fused to a C-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   Hspa8
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_3XHA-ATP6V1B2-mNeonGreen

   Plasmid

   #228865

   Purpose

   Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V1B2 (ATP6V1B2 Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mNeonGreen

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-2dV-Camui (wt)

    Plasmid

    #220366

    Purpose

    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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