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pAG223 PRKD1 3'UTR mut

Plasmid

#12042

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

PRKD1 3'UTR mut (PRKD1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG224 QKI 3'UTR mut

Plasmid

#12043

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

QKI 3'UTR mut (QKI Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pMO14

Plasmid

#235736

Purpose

Expression of human VPS34 complex II in mammalian cells

Depositor

Roger Williams

Article

Mercer et al EMBO J. 2021 Jul 15;40(14):e105985. doi:

Inserts

UVRAG (UVRAG Human)
EGFP
BECN1 (BECN1 Human)
PIK3C3 (PIK3C3 Human)
PIK3R4 (PIK3R4 Human)

Tags

2xStrep-tagII-2xTEV-ZZ

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_GR-RE_MLPmin_BC1092-luc2

Plasmid

#227112

Purpose

Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-NRG1-ScNeo

Plasmid

#209900

Purpose

To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

NRG1-ScNeo (NRG1 Human)

Use

Lentiviral

Tags

mNeonGreen and mScarlet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(D669A)

Plasmid

#220461

Purpose

Express catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Aspartate 669 to alanine

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

Plasmid

#220493

Purpose

To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)

Use

CRISPR

Promoter

U6

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA

Plasmid

#213536

Purpose

Expresses the split transcription factors in mouse liver

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA

Use

AAV

Tags

EGFP and HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23B

Plasmid

#201439

Purpose

Expresses human RAD23B with an N-terminal FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

Plasmid

#160623

Purpose

TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:dCas9:VPR-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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AQTFAATHNPWASQA_epitope

Plasmid

#165019

Purpose

Expression of CMV UL86 (1222-1236) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL86 (1222-1236)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

Plasmid

#160627

Purpose

TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35s-MS2:TV-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJM671 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6 in TUPV3

Plasmid

#161573

Purpose

Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJT43_GalL_BE-P(AP)3

Plasmid

#145036

Purpose

Expresses BE-P(AP)3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-(AP)3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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DKC1 F2.3 gRNA

Plasmid

#90653

Purpose

3rd generation lentiviral gRNA plasmid targeting human DKC1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DKC1 (Guide Designation F2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-DN-CMV-GFP

Plasmid

#83252

Purpose

Adenoviral expression of STAT5A dominant negative with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Mutation

C-terminal deletion of 135 nucleotides (dominant …

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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PPP2R5C B11.5 gRNA

Plasmid

#90853

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R5C

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R5C (Guide Designation B11.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PPP2R2A B5.5 gRNA

Plasmid

#90847

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R2A (Guide Designation B5.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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NAE1 G8.3 gRNA

Plasmid

#90777

Purpose

3rd generation lentiviral gRNA plasmid targeting human NAE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NAE1 (Guide Designation G8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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ANLN A1.2 gRNA

Plasmid

#90530

Purpose

3rd generation lentiviral gRNA plasmid targeting human ANLN

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ANLN (Guide Designation A1.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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