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pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo

Plasmid

#222637

Purpose

Ezrin activation reporter, T567 phosphorylation deficient

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

miRFP680-NES-p2A-ezrin-T567A-mRuby3 (EZR Human)

Use

Lentiviral

Tags

mRuby3

Mutation

T567A

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCI AscI MR1 res

Plasmid

#214752

Purpose

expresses human MR1 resistant to CRISPR editing with a specific sgRNA in mammalian cells

Depositor

David Lewinsohn

Article

Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

Insert

MHC class I-related protein 1 (MR1 Human)

Tags

IRES eGFP

Expression

Mammalian

Mutation

silent mutations to prevent editing by CRISPR/Cas…

Promoter

CMV

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TetOne-Puro-p62 K7A/D69A

Plasmid

#204549

Purpose

Expresses p62 K7A/D69A in a doxycycline-dependent manner in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K7A/D69A

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN

Plasmid

#172435

Purpose

Lentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKL

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
PVR
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

Lentiviral

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

Plasmid

#197454

Purpose

The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 Delta-WD40 propeller (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW& dimerization domain deleted.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NOTCH3(Trunc1)-COMP5AP-AviTag-9xHis

Plasmid

#157609

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NOTCH3 (NOTCH3 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NOTCH3(Trunc2)-COMP5AP-AviTag-9xHis

Plasmid

#157542

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NOTCH3 (NOTCH3 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NOTCH3(Trunc3)-COMP5AP-AviTag-9xHis

Plasmid

#157552

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NOTCH3 (NOTCH3 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NOTCH3(Trunc2)-Fc(DAPA)-AviTag-6xHis

Plasmid

#156978

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NOTCH3 (NOTCH3 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NOTCH3(Trunc3)-Fc(DAPA)-AviTag-6xHis

Plasmid

#156988

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NOTCH3 (NOTCH3 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-BJ.1-AVI

Plasmid

#196617

Purpose

Expresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-BJ.1

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3 Gmut-msfGFP

Plasmid

#180326

Purpose

mammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i3 W502A, H512D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP

Plasmid

#180311

Purpose

bacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Mutation

SEPT2 F20D, V27D

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut1

Plasmid

#180316

Purpose

mammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 W269A, H279D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple-CAAX

Plasmid

#180324

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple and CAAX (HRas)

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3 NCmut-msfGFP

Plasmid

#180325

Purpose

mammalian expression of human SEPT9_i3 NCmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i3 I263D, M270D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1B-FUS-IDR

Plasmid

#183240

Purpose

Expression of HNF1B fused to a intrinsically disordered regions of the FUS protein

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox B (HNF1B Human)

Tags

Fusion to an IDR from the FUS protein and FLAG ta…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1A-delta-IDR2

Plasmid

#183238

Purpose

Expression of FLAG tagged HNF1A with deletion of a intrinsically disordered regions (AA545-573)

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox A (HNF1A Human)

Tags

FLAG tag

Expression

Mammalian

Mutation

Deletion of amino acids 545-573

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 C-V5-tag ORF7a

Plasmid

#179971

Purpose

Mammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 ORF7a (ORF7a Synthetic)

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP7 C-V5-tag

Plasmid

#179963

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126. doi:

Insert

SARS-CoV-1 Nsp7 (ORF1ab Synthetic)

Expression

Mammalian

Mutation

human codon-optimized

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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