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GFP-GW1D10(aa566-1343)

Plasmid

#21517

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa566-1343

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
GFP-GW1D5(aa1670-1962)

Plasmid

#21519

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa1670-1962

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

Plasmid

#191500

Purpose

GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Creation of transgenic fish using tol2 transgenes…

Promoter

myo6b

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1-GFP (A317-318; A341)

Plasmid

#204198

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

3xHA, eGFP and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(AtUBI10)

Plasmid

#202016

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Tags

GB1 and sfGFP

Expression

Plant

Promoter

AtUBI10

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-mScarlet

Plasmid

#198507

Purpose

Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR-attL5-CRY2-mCh-attL2

Plasmid

#160438

Purpose

Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

Plasmid

#154303

Purpose

Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Inserts

histone H1
γ-tubulin

Use

Dictyostelium expression

Tags

mEGFP and mRuby3

Promoter

actin15

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD

Plasmid

#168153

Purpose

Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS18: pHelper(PmcCAST)_PmcPSP1

Plasmid

#168151

Purpose

Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Mitofusin-1 [N111/24R]

Plasmid

#140072

Purpose

Mammalian Expression Plasmid of anti-Mitofusin-1 (Mouse). Derived from hybridoma N111/24.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Mitofusin-1 (Mouse) recombinant mouse monoclonal antibody (Mfn1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Delta [N151/3.3R]

Plasmid

#114515

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Delta (Rat). Derived from hybridoma N151/3.3.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, Delta (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabrd Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDC183

Plasmid

#106415

Purpose

Co-expresses trans SNARE complex with pQZ210 in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Inserts

SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)

Expression

Bacterial

Mutation

Fragment 141-204, Codon optimization and Fragment…

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRJPaph-mTq2

Plasmid

#67031

Purpose

Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
mTurqoise2

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSR06

Plasmid

#69152

Purpose

read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr II

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0 and codon-optimzed index…

Promoter

rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS

Plasmid

#59388

Purpose

Destination vector with RBPMS for stable cell line generation

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-HIS-Xyl-Xmod-DocIII

Plasmid

#60865

Purpose

Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

Depositor

Hermann Gaub

Article

Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

Inserts

Xyl
Xmod-DocIII

Tags

6xHIS

Expression

Bacterial

Mutation

Threonine 120 to Cysteine in Xylanase domain

Promoter

T7

Available Since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck

Plasmid

#58522

Purpose

Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S)-TS-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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VV246: CoChR(C108S)-GCaMP6f-ER in fck

Plasmid

#58521

Purpose

Expresses CoChR(C108S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

CoChR(C108S)-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 108 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDN-NG1Tt

Plasmid

#44523

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1 (wt) promoter (GAL1 Budding Yeast)
tetR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Promoter

PGal1 (wt)

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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