166,289 results

1. pcU6_3 MS2 sgRNA

   Plasmid

   #92394

   Purpose

   Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chick U6.3

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-SLC22A3_STOP

   Plasmid

   #161190

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A3 (SLC22A3 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

   Plasmid

   #103865

   Purpose

   Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Contains the H133A and H1058A mutations which ina…

   Promoter

   EF1a

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. VV229: GCaMP6f in fck

   Plasmid

   #58514

   Purpose

   Expresses GCaMP6f under the a-CamKII promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   GCaMP6f
   

   Use

   Lentiviral

   Tags

   C-terminal FCYENEV (ER export motif); this was no…

   Expression

   Mammalian

   Promoter

   a-CamKII

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-6xHis-AviTag-ALFAtag-eGFP

   Plasmid

   #195854

   Purpose

   Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   6xHis-AviTag-ALFAtag-eGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG/HA-hBIG1

   Plasmid

   #226253

   Purpose

   Expresses N-terminally HA-tagged hBIG1 in mammalian cells

   Depositor

   Hye-Won Shin

   Insert

   BIG1 (ARFGEF1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. NSUN2

   Plasmid

   #155793

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   NSUN2 (NSUN2 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223-CASK

   Plasmid

   #23470

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   CASK (CASK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pDOE11 Lat52:mCherry transient

   Plasmid

   #124249

   Purpose

   Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.

   Depositor

   Attila Fehér

   Article

   Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. perbB2-ECFP

    Plasmid

    #40268

    Depositor

    Martin Offterdinger

    Article

    Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

    Insert

    erbB2 (ERBB2 Human)
    

    Tags

    ecfp

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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