We narrowed to 10,147 results for: gnas

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pLyGo-Bs-3

Plasmid

#163141

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PamyL-PamyQ-PcryIIIA-cryIIIAstab49

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Bs-2

Plasmid

#163140

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PamyL-PamyQ-PcryIIIA-cryIIIAstab49

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-3

Plasmid

#163129

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-5

Plasmid

#163131

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCASP-3G124-HilA

Plasmid

#153324

Purpose

Shows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

3G124 DARPin but no expression

Use

Salmonella expression

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTAMACHis6_PiuD

Plasmid

#128953

Purpose

expresses PiuD in E.coli

Depositor

James Naismith

Article

Luscher et al Antimicrob Agents Chemother. 2018 May 25

Insert

Putative outer membrane receptor for iron transport (PiuD)

Tags

6xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-105)

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB367

Plasmid

#110277

Purpose

gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

Depositor

Christopher Bahl

Article

Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

Insert

gHEEE_02

Tags

10-His, Tev recognition peptide, and mature DsbC …

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ127B

Plasmid

#99883

Purpose

Express two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Insert

TALE-VP64-T2A-TALE-VP64

Use

TALEN

Expression

Plant

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE NLS

Plasmid

#82465

Purpose

Expresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminus

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Capping Enzyme (Rngtt Mouse)

Tags

Myc

Expression

Mammalian

Mutation

deleted amino acids Nuclear Localization Signal

Promoter

CMV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES-mCE

Plasmid

#82463

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme with Terminal nuclear export signal in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt HIV, Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 STAT1

Plasmid

#67116

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT1 (STAT1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pYNL-C1_PTS1

Plasmid

#65699

Purpose

Expresses YNL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_PTS1

Plasmid

#65700

Purpose

Expresses CNL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pONL-C1_PTS1

Plasmid

#65701

Purpose

Expresses ONL-PTS1 in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 1 (tripeptide SKL)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcdna3 EE Jip1b

Plasmid

#52127

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Tags

EE

Expression

Mammalian

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pET21-MRPL13

Plasmid

#31344

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein L13 mature form (MRPL13 Human)

Tags

His

Expression

Bacterial

Mutation

Mature form after removal of the predicted mitoch…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pCeSR beta deltaN

Plasmid

#13728

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (R186.3 Nematode)

Tags

6xHis

Expression

Bacterial

Mutation

expresses residues 38-240 of C. elegans SRβ with …

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pScSR beta deltaN Flag

Plasmid

#13729

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRP102 Budding Yeast)

Tags

6xHis and Flag

Expression

Bacterial

Mutation

expresses residues 31-244 of S. cerevisiae SRβ wi…

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-PKCsub-RLuc8N-NES

Plasmid

#138213

Purpose

Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

mCherry-PKCsub-RLuc8N-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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anti-FLAG M2 heavy chain

Plasmid

#175359

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 heavy chain

Tags

His8 tag and N-terminal secretion signal peptide …

Expression

Mammalian

Mutation

L51I (see paper)

Promoter

CMV

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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