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pOPTM p62 PB1 (1-122)

Plasmid

#210428

Purpose

Bacterial expression of MBP-PB1(1-122) with TEV cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 PB1 (1-122) (NUP62 Human)

Tags

MBP

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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myr-palm-Gamillus(2x)

Plasmid

#197921

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus-Gamillus

Expression

Mammalian

Mutation

none

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-EE

Plasmid

#198298

Purpose

Produces PVCs retargeted with EE tag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-EE

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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FB430

Plasmid

#203621

Purpose

TU for the expression of phleomycin resistance.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PpcbC:ble:TamdS

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB431

Plasmid

#203622

Purpose

TU for the expression of terbinafine resistance.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PgpdA:ergA:TamdS

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pML32

Plasmid

#203344

Purpose

2-bit register circuit on pLW555 backbone: PTac-S2, PTet-P2, PLux-A1, PLux-H1

Depositor

Lauren Andrews

Article

Lebovich et al ACS Synth Biol. 2023 Aug 15. doi: 10.102

Inserts

srpR
phlF
amtR
hlyIIR

Promoter

PAmtR and PTet, PPhlF and PLux*, PSrpR and PLux*,…

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH-pnRFP-B30Y

Plasmid

#201823

Purpose

Expresses pnRFP-B30Y in mammalian cells

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

pnRFP-B30Y

Expression

Mammalian

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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GPI-mGreenLantern(2x)

Plasmid

#197917

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mGreenLantern-mGreenLantern

Expression

Mammalian

Mutation

none

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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GPI-Gamillus(2x)

Plasmid

#197915

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus-Gamillus

Expression

Mammalian

Mutation

none

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-degFusionRed

Plasmid

#199463

Purpose

Arabinose-induced expression of the photodegradable His-tagged protein degFusionRed in E. coli.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

degFusionRed

Tags

6xHis

Expression

Bacterial

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-Gamillus

Plasmid

#197920

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus

Expression

Mammalian

Mutation

none

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvH-CtNot1_925-2193_X

Plasmid

#147972

Purpose

Bacterial Expression of CtNot1_925-2193

Depositor

Elisa Izaurralde

Article

Raisch et al J Struct Biol. 2018 Dec;204(3):388-395.

Insert

CtNot1_925-2193

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pNL-5.1

Plasmid

#190589

Purpose

Lentiviral vector-mediated delivery of artificial exons for protein tagging in mammalian cells

Depositor

Jakob Reiser

Article

Bialkowska et al BMC Genomics. 2005 Sep 4;6:113. doi: 10.

Inserts

NeoR transgene
artificial exon encoding BSD and EGFP

Use

Lentiviral

Promoter

Cellular and SV40

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pnEK-NvH-DmeIF4E_Z

Plasmid

#148195

Purpose

Bacterial Expression of DmeIF4E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4E

Expression

Bacterial

Mutation

3 silent mutations compared to the sequence given…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnEK-NvSHN-DmeIF4E_Z

Plasmid

#148198

Purpose

Bacterial Expression of DmeIF4E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4E

Expression

Bacterial

Mutation

3 silent mutations compared to the sequence given…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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SpAGS-dGoLoco1-GFP

Plasmid

#185577

Purpose

for overexpression

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-dGoLoco1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

deletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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LDB73

Plasmid

#185437

Purpose

NUP1 CEN URA3 vector for synthetic lethal sectoring assays and screening

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

Insert

NUP1

Expression

Yeast

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-matDYK

Plasmid

#115258

Purpose

For mammalian expression of the human NODAL open reading frame with an internal DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-T7TS

Plasmid

#115250

Purpose

For in vitro transcription of human NODAL open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMA3998rc

Plasmid

#184851

Purpose

A retroviral vector with blasticidin resistance for cloning inserts between loxP sites

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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