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We narrowed to 10,501 results for: ada

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Showing: 8361 - 8380 of 10501 results

  4. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid
    #185489
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185489-plasmid-map-sequence-id-362674

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  7. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

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  12. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid
    #223125
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223125-plasmid-map-sequence-id-447703

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  14. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Qiao Zhou
    Article
    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61041-plasmid-map-sequence-id-456476

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  15. pLXSN p110 CUX1

    Plasmid
    #90471
    Purpose
    Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively
    Depositor
    Alain Nepveu
    Article
    Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11
    Insert
    CUX1 (amino acids 747-1505) (CUX1 Human)
    Use
    Retroviral
    Tags
    HA and Myc
    Expression
    Mammalian
    Promoter
    Moloney murine leukemia virus long terminal repeat
    Available Since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    90471-plasmid-map-sequence-id-205963

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  16. pGL4.23 C5_15

    Plasmid
    #60322
    Purpose
    This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.
    Depositor
    Jorge Ferrer
    Article
    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1
    Insert
    Non active element (nearest TSS PHLDA1) (PHLDA1 Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Available Since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pCW-eGFP-FHA

    Plasmid
    #114129
    Purpose
    Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin
    Depositor
    Daniel Durocher
    Article
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Insert
    RNF8 FHA domain (amino acids 2-160) (RNF8 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    aa 2-160
    Promoter
    TRE promoter, Tet ON
    Available Since
    Aug. 28, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114129-plasmid-map-sequence-id-220207

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Showing: 8361 - 8380 of 10501 results