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pHR- flag-Lag17-iCAM-1

Plasmid

#205204

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG17

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag17 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-DLL1

Plasmid

#205201

Purpose

This vector encodes of the synCAM (DLL-1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM DLL1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-MUC4

Plasmid

#205198

Purpose

This vector encodes of the synCAM (MUC4) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM MUC4 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P2

Plasmid

#194344

Purpose

sgRNA position entry clone 2

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P1

Plasmid

#194343

Purpose

sgRNA position entry clone 1

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P3

Plasmid

#194345

Purpose

sgRNA position entry clone 3

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P4

Plasmid

#194346

Purpose

sgRNA position entry clone 4

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P5

Plasmid

#194347

Purpose

sgRNA position entry clone 5

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P6

Plasmid

#194348

Purpose

sgRNA position entry clone 6

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pECNUS_P7

Plasmid

#194349

Purpose

sgRNA position entry clone 7

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

sgRNA expression cassettes and LacZ

Expression

Plant

Promoter

Ath U6 promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No3

Plasmid

#194898

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-3 targeting the promoter of human MECP2

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.3

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No9

Plasmid

#194903

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-9 targeting the promoter of human MECP2.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.9

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSLiP-G1

Plasmid

#188963

Purpose

L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

dCas9
sgRNA: atcggtcgcattgttttccactagg

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-fibcon-iCAM1

Plasmid

#205212

Purpose

This vector encodes of the synCAM (fibcon - iCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM fibcon - iCAM1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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phCMV-IE1

Plasmid

#118048

Purpose

GBPart - Cytomegalovirus enhancer and promoter for constitutive high level expression in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Cytomegalovirus enhancer and promoter

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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FRT2-H2B-EGFP-V5-2A (IG157)

Plasmid

#99623

Purpose

To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pafG-CBE-3T

Plasmid

#220374

Purpose

for hA3A-nCas9 and gRNA expression in Aspergillus flavus

Depositor

Xue Gao

Article

Zhao et al J Am Chem Soc. 2023 Jan 11;145(1):413-42

Inserts

hA3A-nCas9
tRNA and gRNA scaffold

Use

CRISPR

Promoter

Ptef1 and tRNA

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-Y132D

Plasmid

#193641

Purpose

Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-Y132D

Expression

Mammalian

Mutation

hAPOBEC3A-Y132D

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLucia

Plasmid

#135287

Purpose

GBPart - Lucia Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Lucia Luciferase

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-N57G

Plasmid

#193639

Purpose

Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-N57G, EGFP

Expression

Mammalian

Mutation

hAPOBEC3A-N57G

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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