1,862 results

1. pGC302

   Plasmid

   #19668

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   <*iA-4xNLS-mCherry::let-858(3’)
   

   Use

   Flp/frt

   Tags

   4xNLS

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pOX + kqt2

   Plasmid

   #16137

   Depositor

   Larry Salkoff

   Article

   Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

   Insert

   Caenorhabditis elegans potassium channel (kqt-2 Nematode)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   Deletion of aa1-38 compared to GenBank NP_509392.3

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

3. TU#1450_unc-53a_cDNA

   Plasmid

   #82361

   Purpose

   express unc-53a cDNA

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   unc-53 a isoform (unc-53 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   none

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOX + nSlo2

   Plasmid

   #16202

   Depositor

   Larry Salkoff

   Article

   Yuan et al Nat Neurosci. 2000 Aug . 3(8):771-9.

   Insert

   Caenorhabditis elegans SLOwpoke potassium channel (slo-2 Nematode)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pCM1.112

   Plasmid

   #19599

   Depositor

   Geraldine Seydoux

   Insert

   fbf-2 prom in PDONRP4P1R
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Nov. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNM3746

   Plasmid

   #154109

   Purpose

   loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Inserts

   LoxP-SEC-LoxP
   FRT::rpl-28p::GFP::His-58::FRT3
   mex-5p::FLP D5::sl2::mNG::glh2 3'
   

   Use

   Cre/Lox; Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGC193

   Plasmid

   #19686

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   hlh-12(R25K)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGC310

   Plasmid

   #19669

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   <*iA-1xNLS-mCherry::let-858(3’)
   

   Use

   Flp/frt

   Tags

   1xNLS

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. JH05

   Plasmid

   #73516

   Purpose

   mYPet_PH pCFJ150 - contain C. elegans codon optimized mYPet

   Depositor

   Bob Goldstein

   Article

   Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

   Insert

   pmex-5::mPYet::PH::tbb-2
   

   Use

   Tags

   mYPet

   Expression

   Mutation

   Promoter

   mex-5

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCM5.46

    Plasmid

    #19386

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    pgl-3 3'utr in pDONRP2RP3 (pgl-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIK137

    Plasmid

    #65632

    Purpose

    Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Insert

    Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEV9.03

    Plasmid

    #37495

    Depositor

    Geraldine Seydoux

    Article

    Voronina et al Development. 2010 May;137(9):1441-50. Ep

    Insert

    npp-16 (npp-16 Nematode)
    

    Use

    RNAi

    Tags

    Expression

    Worm

    Mutation

    nts 162-673 of cds

    Promoter

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pLexA::nud-1

    Plasmid

    #11339

    Depositor

    Guy Caldwell

    Insert

    nud-1 (nud-1 Nematode)
    

    Use

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pLexA::lis-1

    Plasmid

    #11338

    Depositor

    Guy Caldwell

    Insert

    lis-1 (lis-1 Nematode)
    

    Use

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pMLS285

    Plasmid

    #73734

    Purpose

    SapTrap egl-13 NLS connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    egl-13 NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pClneoMyc' CST-1

    Plasmid

    #66859

    Purpose

    Express Myc-tagged C.elegans CST-1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

    Insert

    CST-1 (cst-1 Nematode)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S48N, H50R, S257P and V340A mutations in CST-1 co…

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pClneoFH-let60 G12V

    Plasmid

    #66860

    Purpose

    Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

    Insert

    Let-60 (let-60 Nematode)
    

    Use

    Tags

    FLAG-His6

    Expression

    Mammalian

    Mutation

    Glycine 12 is mutated to valine

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159848

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159856

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TU#1806_CRISPR_unc-73_exon21

    Plasmid

    #82360

    Purpose

    to create unc-73E null allele

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge