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pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

Plasmid

#159802

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPV454

Plasmid

#42922

Depositor

James Lok

Article

Stoltzfus et al PLoS One. 2012;7(6):e38587. doi: 10.1371

Insert

Ce-age-1 promoter (873 bp)::Ce-age-1 cDNA (3549 bp)::Ce-unc-54 terminator (765 bp) (age-1 Nematode)

Expression

Worm

Promoter

Ce-age-1 promoter (873 bp)

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pJM601-TRA-2::FLAG

Plasmid

#27129

Depositor

Ding Xue

Article

Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

Insert

TRA-2 FLAG (tra-2 Nematode)

Tags

3x FLAG

Expression

Worm

Mutation

Replaced TRA-2 stop codon with KpnI and EcoRI and…

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::mCherry

Plasmid

#124348

Purpose

Nuclear Localized Pan-Neuronal mCherry

Depositor

Andrew Leifer

Insert

rab-3, mCherry (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS2-his-24::tagRFP

Plasmid

#124330

Purpose

Expressing Nuclear Localized tagRFP

Depositor

Andrew Leifer

Insert

tagRFP (his-24 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSR28

Plasmid

#69253

Purpose

phlh-8::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

hlh-8

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ2453 - [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)

Plasmid

#159863

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCM1.157

Plasmid

#21388

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

gld-1/cye-1 operon in pDONRP2RP3

Use

Gateway

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pJW1328

Plasmid

#69488

Purpose

sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans

Depositor

Jordan Ward

Insert

sgRNA(F+E) targeting pha-1

Expression

Worm

Promoter

R07E5.16 U6 promoter

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pEL392

Plasmid

#52348

Purpose

GFP reporter for fli-1

Depositor

Erik Lundquist

Article

Lu et al BMC Dev Biol. 2008 May 16;8:54. doi: 10.

Insert

fli-1 (fli-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

fli-1

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pJRK86

Plasmid

#173743

Purpose

general AID::GFP expression

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::AID::GFP::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGC302

Plasmid

#19668

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-4xNLS-mCherry::let-858(3’)

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pOX + kqt2

Plasmid

#16137

Depositor

Larry Salkoff

Article

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-2 Nematode)

Use

Xenopus oocyte expression

Mutation

Deletion of aa1-38 compared to GenBank NP_509392.3

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pCM3.23

Plasmid

#21389

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

gld-1 ORF+3' in pDONR201 (gld-1 Nematode)

Use

Gateway

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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frh-1-B

Plasmid

#15400

Depositor

Michael Ristow

Article

Zarse et al FASEB J. 2007 Apr . 21(4):1271-5.

Insert

Frataxin homologue (frh-1 Nematode)

Use

RNAi

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pOX + nSlo2

Plasmid

#16202

Depositor

Larry Salkoff

Article

Yuan et al Nat Neurosci. 2000 Aug . 3(8):771-9.

Insert

Caenorhabditis elegans SLOwpoke potassium channel (slo-2 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
TU#1450_unc-53a_cDNA

Plasmid

#82361

Purpose

express unc-53a cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-53 a isoform (unc-53 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ2262A - [1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159851

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mNeon (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCM1.112

Plasmid

#19599

Depositor

Geraldine Seydoux

Insert

fbf-2 prom in PDONRP4P1R

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pGC310

Plasmid

#19669

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*iA-1xNLS-mCherry::let-858(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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