2,223 results

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pDONR_P2R-P3_3'arm_apobb.1

Plasmid

#128681

Purpose

apobb.1 homology arm 3' gateway clone

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

apobb.1 (apobb.1 Zebrafish)

Use

Multi-step gateway cloning

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoEa

Plasmid

#121900

Purpose

Plasmid for making zebrafish apoEa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoEa (apoea Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pT2-bactin:QF2,crybb:CFP

Plasmid

#125697

Purpose

Expresses QF2 ubiquitously in zebrafish, with selectable marker CFP in eye lens

Depositor

Daniel Gorelick

Insert

bactin:QF2

Use

Zebrafish expression

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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kank3_pJet1.2

Plasmid

#121981

Purpose

for generating kank3 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank3 (kank3 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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kank4_pJet1.2

Plasmid

#121982

Purpose

for generating kank4 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank4 (kank4 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

Plasmid

#119074

Purpose

Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:MCP-p65-HSF1-2a-Cerulean

Expression

Bacterial

Promoter

Zebrafish ubiquitin promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-Ds

Plasmid

#119069

Purpose

To clone sgRNA spacer sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

Plasmid

#119075

Purpose

Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:Com-KRAB-2a-Cerulean

Expression

Bacterial

Promoter

Zebrafish ubiquitin promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-com-Ds

Plasmid

#119077

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xCom-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-MS2-Ds

Plasmid

#119076

Purpose

To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:2xMS2-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-UAS-Dendra2-miR-223 sponge

Plasmid

#97146

Purpose

For miR-223 knock down

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223 sponge

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-krt4-RFP-miR-223 sponge

Plasmid

#97150

Purpose

For miR-223 knock down

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223 sponge

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-ndufs2 guide

Plasmid

#107601

Purpose

An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

ndufs2 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E DN Kif5a

Plasmid

#105973

Purpose

3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Kif5a (kif5aa Zebrafish)

Expression

Bacterial

Mutation

truncation of aa 416-1033

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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p3E NWASP-CA

Plasmid

#105968

Purpose

3' element for Gateway cloning, NWASP C terminal domain

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NWASP-CA

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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ptdTomato-N1-CMVL-ikk1FL(C179A)-tdTomato

Plasmid

#105644

Purpose

Zebrafish Inhibitor of kappa B kinase alpha with mutation at position 179 from cysteine to alanine

Depositor

Sandra Rieger

Article

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Insert

CMVL-ikk1FL(C179A)-tdTomato (chuk Zebrafish)

Tags

tdTomato

Expression

Mammalian

Mutation

CMV:ikk1FL(C178A)-tdTomato

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only
pT2KXIG (Tol2)-tp63:ikk1C179A:GFP

Plasmid

#105647

Purpose

Expression of Ikk1(alpha) with mutated cysteine residues at position 179 in basal keratinocytes

Depositor

Sandra Rieger

Article

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Insert

tp63:ikk1C179A:GFP (chuk Zebrafish)

Tags

GFP

Expression

Mammalian

Mutation

p63 promoter + ikk1C179A + GFP

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 2kb promoter/AcGFP

Plasmid

#98099

Purpose

Zebrafish aBb crystallin 2kb promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Danio alpha Ba-crystallin 3kb promoter/AcGFP

Plasmid

#98097

Purpose

aBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Ba-crystallin 3 kb fragment (-3000/-1)

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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p5Ecldn5bE1

Plasmid

#90113

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of cldn5b

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

cldn5bE1 (cldn5b Zebrafish)

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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