We narrowed to 7,571 results for: Lif

1. rpaA_NS1

   Plasmid

   #101829

   Purpose

   drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   RpaA with its native promoter
   

   Use

   Cyanobacteria cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. P0050::rpaA

   Plasmid

   #101830

   Purpose

   drives expression of rpaA from an rpaA-independent promoter

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   RpaA with p0050 promoter (+ gentamicin resistance casette)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ptrc::galP_NS1

   Plasmid

   #101843

   Purpose

   drives expression of galP transporter from NS1 locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   Ptrc promoter driving GalP transporter expression
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. C064

   Plasmid

   #174353

   Purpose

   Plasmid for expressing frog PABPC1 (C-His) in E.coli

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   PABPC1 (pabpc1.S Frog)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG/MNase

   Plasmid

   #123461

   Purpose

   Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

   Depositor

   Steven Henikoff

   Article

   Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

   Insert

   Protein A-Protein G-Micrococcal Nuclease
   

   Use

   Tags

   6xHIS and HA

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK231 (AAVS1 CMV-MCS-PURO)

   Plasmid

   #105924

   Purpose

   Donor for integration at the human AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. hROSA26 CRISPR-pX330

   Plasmid

   #105927

   Purpose

   hROSA26 targeting CRISPR plasmid

   Depositor

   Masato Kanemaki

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   hROSA26 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-TRE-dTomato-GCaMP6s

   Plasmid

   #99864

   Purpose

   expresses dTomato and GCaMP6s under control of the Tet-off system

   Depositor

   Venkatesh Murthy

   Article

   Wallace et al Elife. 2020 Mar 9;9. pii: 50531. doi: 10

   Insert

   dTomato, GCaMP6s
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Tet-responsive promoter

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. epB_CAG_TetRFlag-nls-GFPx2_DEx2

   Plasmid

   #119910

   Purpose

   ePiggyBac mammalian expression plasmid for TetR-GFP fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   TetRFlag-nls-GFPx2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRX-No-HaloTag7-mGFP-LC3-mRFP

    Plasmid

    #184902

    Purpose

    Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7, monomeric EGFP, and monomeric RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQIq_MRGS_HIS8_(27F3)_FLAG

    Plasmid

    #199615

    Purpose

    Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG 27F3
    

    Use

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-mCherry-CDK4KTR

    Plasmid

    #126680

    Purpose

    Fluorescent reporter for CDK4/6 activity

    Depositor

    Hee Won Yang

    Article

    Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

    Insert

    RB cterm
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-U6-gRNA-EF1a-EGFP-CAAX

    Plasmid

    #167936

    Purpose

    gRNA entry vector

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    U6-gRNA backbone
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-mCherry

    Plasmid

    #197420

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPBpuro-hM3D-P2Av3-mCherryNLS

    Plasmid

    #184714

    Purpose

    Gq DREADD

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    hM3D
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. GAL4BD-VP16/pDON207

    Plasmid

    #201230

    Purpose

    GAL4-VP16 transcription factor

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    GAL4BD-VP16
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. N1-G-Ca-FLITS

    Plasmid

    #191465

    Purpose

    Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACS129

    Plasmid

    #204594

    Purpose

    Histone H3.1-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    histone H3.1 (H3C3 Human)
    

    Use

    Lentiviral

    Tags

    human histone H3.1-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2(+)-zGrad

    Plasmid

    #119716

    Purpose

    Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

    Depositor

    Holger Knaut

    Article

    Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

    Insert

    zGrad
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1-jGCaMP7b-XC

    Plasmid

    #178361

    Purpose

    Improved genetic encoded calcium sensor based on GCaMP7b.

    Depositor

    Xiaodong Liu

    Article

    Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

    Insert

    jGCaMP7b-XC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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