We narrowed to 24,438 results for: Nes;

1. pGI3EM20C

   Plasmid

   #135487

   Purpose

   Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

   Depositor

   Nicolas Buchler

   Article

   Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

   Insert

   hph SpunH2A/Bpr H2B-tdTomato
   

   Use

   Tags

   tdTomato

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpunH2A/Bpr (divergent)

   Available since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSimpleII-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47867

   Purpose

   This plasmid encode NmCas9 with two NLS and a HA tag

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. EcPPK1

   Plasmid

   #108850

   Purpose

   Expresses E. coli PPK1 in mammalian cell lines

   Depositor

   Michael Downey

   Article

   Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

   Insert

   Polyphosphate Kinase 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM33_PEX14_Pex14

   Plasmid

   #178484

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   PEX14_Pex14 (PEX14 Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM33_OXSR1_CCT

   Plasmid

   #178482

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   OXSR1_OXSR1 (OXSR1 Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNB785

   Plasmid

   #60164

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   SIC1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB787

   Plasmid

   #60152

   Purpose

   Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LEU1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNB789

   Plasmid

   #60166

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   RNR1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Human Mevalonate diphosphate decarboxylase (MDD)

   Plasmid

   #52431

   Purpose

   bacterial expression of human Mevalonate diphosphate decarboxylase (MDD)

   Depositor

   Henry Miziorko

   Article

   Voynova et al Arch Biochem Biophys. 2008 Dec 1;480(1):

   Insert

   mevalonate diphosphate decarboxylase (MVD Human)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pLentiRNACRISPR_010-TetO-NLS-RfxCas13d-2A-mCherry-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228555

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    RfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA and P2A-mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228557

    Purpose

    Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    dRfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228558

    Purpose

    Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    dhfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWN103

    Plasmid

    #233860

    Purpose

    MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWN104

    Plasmid

    #233861

    Purpose

    MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-Syn1-mCherry

    Plasmid

    #222967

    Purpose

    Make lentivirus to express mCherry in syn1 expressing cells

    Depositor

    Xinyu Zhao

    Article

    Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    synapsin I

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM33_SPSB1_SPRY

    Plasmid

    #178487

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    SPSB1_SPRY (SPSB1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM33_YES1_SH3

    Plasmid

    #178492

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    YES1_SH3 (YES1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-TREtight-H2b-emiRFP670-P2A-TEVP

    Plasmid

    #174379

    Purpose

    AAV virus for expressing TEVP and H2b-emiRFP670

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    TEVP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRV∆G-NPEST-4Cre

    Plasmid

    #174380

    Purpose

    Rabies vector for expressing Cre recombinase and N gene with PEST domain

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    Cre recombinase
    

    Use

    Rabies

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #138149

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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