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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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Hsh155 K740R

Plasmid

#111953

Purpose

This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K740R mutation

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 K740R

Expression

Bacterial and Yeast

Mutation

K740R

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 STAT6

Plasmid

#67123

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT6 (STAT6 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFDP3

Plasmid

#67083

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP3 (TFDP3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFDP1

Plasmid

#67084

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP1 (TFDP1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFDP2

Plasmid

#67088

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP2 (TFDP2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TFF1

Plasmid

#67047

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFF1 (TFF1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
CNTL-sfGFP

Plasmid

#240100

Purpose

SP6-GFP plasmid

Depositor

Genevieve Kendall

Article

Kucinski et al Cell Rep. 2025 Jul 22;44(7):115923. doi:

Insert

sfGFP

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1)

Plasmid

#233273

Purpose

To express GFP from a CMV promoter and to express an shRNA targeting Rat Nurr1

Depositor

Jonathan Ploski, Glenn Schafe

Insert

GFP and shRNA targeting Rat Nurr1 (Nr4a2 Rat)

Use

AAV

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #5)

Plasmid

#233274

Purpose

To express GFP from a CMV promoter and to express an shRNA targeting Rat Nurr1

Depositor

Jonathan Ploski, Glenn Schafe

Insert

GFP and shRNA targeting Rat Nurr1 (Nr4a2 Rat)

Use

AAV

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR2 long (1-393)

Plasmid

#236270

Purpose

Bacterial expression of bovine arrestin-2 long isoform (1-393) Q394Stop

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-2 (ARRB2 Bovine)

Expression

Bacterial

Mutation

long, (1-393) Q394Stop

Promoter

lac

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR2 long (1-382)

Plasmid

#236269

Purpose

Bacterial expression of bovine arrestin-2 long isoform (1-382) D383Stop

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-2 (ARRB2 Bovine)

Expression

Bacterial

Mutation

long, (1-382) D383Stop

Promoter

lac

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR2 short

Plasmid

#236267

Purpose

Bacterial expression of bovine arrestin-2 short isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-2 (ARRB2 Bovine)

Expression

Bacterial

Mutation

short isoform

Promoter

lac

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-FLAG-mouse Beta 2 Adrenergic Receptor (Adrb2)

Plasmid

#233041

Purpose

To Express a CRE dependent Flag tagged Mouse Beta 2 Adrenergic Receptor (Adrb2) from an EF1a promoter

Depositor

Jonathan Ploski

Insert

mouse Beta 2 Adrenergic Receptor (Adrb2 Mouse)

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-LOVPLD-mCherry-OSBP(PH)

Plasmid

#219561

Purpose

Golgi-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

LOVPLD-mCherry-OSBP(PH)

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Expression

Mammalian

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2

Plasmid

#191320

Purpose

Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-Dronpa

Plasmid

#191321

Purpose

Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-Dronpa (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP229-Lenti-TRE3G-4xMyc-GluN2B-WPRE-pA

Plasmid

#164794

Purpose

pLenti vector backbone designed to express 4XMyc Tagged-GluN2B from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

4xMyc

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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