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Plasmid#203880PurposepHI1 with insertion of ampicillin-resistance geneDepositorInsertbroad-spectrum class A beta-lactamase TEM-116
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9CR4-NG
Plasmid#120225PurposepCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.DepositorInsertSpCas9-NG
UseSynthetic BiologyTagsssrAExpressionMutationMutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…PromoterpTetAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS169
Plasmid#194492PurposetCYC1 (Part 4)DepositorInserttCYC1 (Part 4)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBCN22-R4R3
Plasmid#26302DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBTL-3
Plasmid#22807DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBCN21-R4R3
Plasmid#26301DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTKIP-cat
Plasmid#41065DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBT-3
Plasmid#22832DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCas9CR4-VQR
Plasmid#120224PurposepCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015DepositorInsertSpCas9-VQR
UseSynthetic BiologyTagsssrAExpressionMutationMutations D1135V/R1335V/T1337RPromoterpTetAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMT-3
Plasmid#22838DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBMTL-3
Plasmid#22813DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBCN24-R4R3
Plasmid#26304DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBMTB-3
Plasmid#22826DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBCN28-R4R3
Plasmid#26303DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBCN27-R4R3
Plasmid#26346DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBCN25-R4R3
Plasmid#26305DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_LEU2_I
Plasmid#109060Purposeyeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposesDepositorInsertLEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterLEU2Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ubi:ccdb + mcherry
Plasmid#233303PurposeGATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and mCherry markerDepositorInsertUbi:ccdb + mcherry
UseGateway destinationTagsExpressionMutationPromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
DEXsynth:ccdb+ RUBY
Plasmid#233320PurposeGATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of ccdb selection toxin and RUBY markerDepositorInsertDEXsynth:ccdb+ RUBY
UseGateway destinationTagsExpressionMutationPromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
DEXsynth:ccdb+ mcherry
Plasmid#233315PurposeGATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of ccdb selection toxin and mCherry markerDepositorInsertDEXsynth:ccdb+ mcherry
UseGateway destinationTagsExpressionMutationPromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only